M00052-3

antibody from Boster Biological Technology

Targeting: CD44 CD44R, CSPG8, HCELL, IN, MC56, MDU2, MDU3, MIC4, Pgp1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

M00052-3

Provider

Boster Biological Technology

Product name

Anti-CD44 Picoband™ Antibody (monoclonal, 7H7)

Antibody type

Monoclonal

Description

Mouse IgG monoclonal antibody for CD44 detection. Tested with WB, ICC/IF, FCM in Human;Rat.

Reactivity

Human, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

7H7

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

Differentiation of Bone Mesenchymal Stem Cells Into Vascular Endothelial Cell-Like Cells Using Functionalized Single-Walled Carbon Nanotubes.

Luo F, Li R, Zheng H, Xu Y, Yang L, Qu C, Hong G, Wan Q
Frontiers in bioengineering and biotechnology 2022;10:913080

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Overexpression of microRNA-205-5p exerts suppressive effects on stem cell drug resistance in gallbladder cancer by down-regulating PRKCE.

Zhang GF, Wu JC, Wang HY, Jiang WD, Qiu L
Bioscience reports 2020 Sep 30;40(9)

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Protective Mechanism of the Antioxidant Baicalein toward Hydroxyl Radical-Treated Bone Marrow-Derived Mesenchymal Stem Cells.

Tian Y, Li X, Xie H, Wang X, Xie Y, Chen C, Chen D
Molecules (Basel, Switzerland) 2018 Jan 20;23(1)

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Antioxidant and Cytoprotective Effects of the Di-O-Caffeoylquinic Acid Family: The Mechanism, Structure-Activity Relationship, and Conformational Effect.

Li X, Li K, Xie H, Xie Y, Li Y, Zhao X, Jiang X, Chen D
Molecules (Basel, Switzerland) 2018 Jan 20;23(1)

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Two phenolic antioxidants in Suoyang enhance viability of •OH-damaged mesenchymal stem cells: comparison and mechanistic chemistry.

Xie Y, Li X, Xu J, Jiang Q, Xie H, He J, Chen D
Chemistry Central journal 2017 Aug 25;11(1):84

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Differentiation of mesenchymal stem cells into neural stem cells using cerebrospinal fluid.

Ge W, Ren C, Duan X, Geng D, Zhang C, Liu X, Chen H, Wan M, Geng R
Cell biochemistry and biophysics 2015 Jan;71(1):449-55

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Overexpression of inducible nitric oxide synthase impairs the survival of bone marrow stem cells transplanted into rat infarcted myocardium.

Li HM, Liu L, Mei X, Chen H, Liu Z, Zhao X
Life sciences 2014 Jun 13;106(1-2):50-7

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Colorectal cancer progression is associated with accumulation of Th17 lymphocytes in tumor tissues and increased serum levels of interleukin-6.

Li X, Wang Y, Han C, Li P, Zhang H
The Tohoku journal of experimental medicine 2014 Jul;233(3):175-82

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Folium Sennae protects against hydroxyl radical-induced DNA damage via antioxidant mechanism: an in vitro study.

Lin J, Li X, Han L, Li F, Lu W, Bai Y, Chen D
Botanical studies 2014 Dec;55(1):16

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Genistein promotion of osteogenic differentiation through BMP2/SMAD5/RUNX2 signaling.

Dai J, Li Y, Zhou H, Chen J, Chen M, Xiao Z
International journal of biological sciences 2013;9(10):1089-98

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HER2 interacts with CD44 to up-regulate CXCR4 via epigenetic silencing of microRNA-139 in gastric cancer cells.

Bao W, Fu HJ, Xie QS, Wang L, Zhang R, Guo ZY, Zhao J, Meng YL, Ren XL, Wang T, Li Q, Jin BQ, Yao LB, Wang RA, Fan DM, Chen SY, Jia LT, Yang AG
Gastroenterology 2011 Dec;141(6):2076-2087.e6

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Prognostic significance of Survivin and CD44v6 in laryngeal cancer surgical margins.

Zhao H, Ren J, Zhuo X, Ye H, Zou J, Liu S
Journal of cancer research and clinical oncology 2008 Oct;134(10):1051-8

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Suspension culture combined with chemotherapeutic agents for sorting of breast cancer stem cells.

Li HZ, Yi TB, Wu ZY
BMC cancer 2008 May 14;8:135

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Articular cartilage metabolism in patients with Kashin-Beck Disease: an endemic osteoarthropathy in China.

Cao J, Li S, Shi Z, Yue Y, Sun J, Chen J, Fu Q, Hughes CE, Caterson B
Osteoarthritis and cartilage 2008 Jun;16(6):680-8

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Inhibitory effect of mesenchymal stem cells on lymphocyte proliferation.

Gao K, Chen Y, Wei L, Li S, Jin X, Cong C, Yuan Y, Long D, Li Y, Cheng J, Lu Y
Cell biochemistry and function 2008 Dec;26(8):900-7

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Effects of body-resistance strengthening and tumor-suppressing granules on immune adhesion function of red blood cells and expression of metastasis protein CD44 in tumor cells of patients with esophageal carcinoma.

Zhao JX, Li XF, Wang XX
World journal of gastroenterology 2007 Aug 28;13(32):4360-4

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of CD44 using anti-CD44 antibody (M00052-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat C6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- CD44 antigen affinity purified monoclonal antibody (Catalog # M00052-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CD44 at approximately 82KD. The expected band size for CD44 is at 82KD.

Additional image