60224-1-Ig

antibody from Proteintech Group

Targeting: CD44 CD44R, CSPG8, HCELL, IN, MC56, MDU2, MDU3, MIC4, Pgp1

Provider product page for 60224-1-Ig

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [46]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 60224-1-Ig - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#60224-1-Ig, RRID:AB_11042767
Product name: CD44 antibody
Antibody type: Monoclonal
Description: KD/KO validated CD44 antibody (Cat. #60224-1-Ig) is a mouse monoclonal antibody that shows reactivity with human, pig and has been validated for the following applications: FC, IF, IHC, WB,ELISA.
Reactivity: Human, Porcine
Host: Mouse
Conjugate: Unconjugated
Isotype: IgG
Antibody clone number: 6F4H2
Vial size: 20ul, 150ul

CD44 protein structure - 60224-1-Ig shown in red.

Submitted references Hypoxia-induced TREM1 promotes mesenchymal-like states of glioma stem cells via alternatively activating tumor-associated macrophages.

Dong M, Zhang X, Peng P, Chen Z, Zhang Y, Wan L, Xiang W, Liu G, Guo Y, Xiao Q, Wang B, Guo D, Zhu M, Yu X, Wan F
Cancer letters 2024 May 28;590:216801

EphA2 promotes the transcription of KLF4 to facilitate stemness in oral squamous cell carcinoma.

Bai J, Chen Y, Sun Y, Wang X, Wang Y, Guo S, Shang Z, Shao Z
Cellular and molecular life sciences : CMLS 2024 Jun 25;81(1):278

Lactate-driven type I collagen deposition facilitates cancer stem cell-like phenotype of head and neck squamous cell carcinoma.

Sun Y, Chen Y, Zhao H, Wang J, Liu Y, Bai J, Hu C, Shang Z
iScience 2024 Apr 19;27(4):109340

Decellularised spinal cord matrix manipulates glial niche into repairing phase via serglycin-mediated signalling pathway.

Zhang S, Zhai M, Xu Y, Han J, Chen J, Xiong Y, Pan S, Wang Q, Yu C, Rao Z, Sun Q, Sui Y, Fan K, Li H, Guo W, Liu C, Bai Y, Zhou J, Quan D, Zhang X
Cell proliferation 2023 Sep;56(9):e13429

Mesenchymal stem cells in synovial fluid increase in number in response to synovitis and display more tissue-reparative phenotypes in osteoarthritis.

Furuoka H, Endo K, Sekiya I
Stem cell research & therapy 2023 Sep 8;14(1):244

Lgr5-expressing secretory cells form a Wnt inhibitory niche in cartilage critical for chondrocyte identity.

Ruscitto A, Chen P, Tosa I, Wang Z, Zhou G, Safina I, Wei R, Morel MM, Koch A, Forman M, Reeve G, Lecholop MK, Wilson M, Bonthius D, Chen M, Ono M, Wang TC, Yao H, Embree MC
Cell stem cell 2023 Sep 7;30(9):1179-1198.e7

Local transplantation of mesenchymal stem cells improves encephalo-myo-synangiosis-mediated collateral neovascularization in chronic brain ischemia.

Zhang X, Huang Y, Liu Y, Liu Y, He X, Ma X, Gan C, Zou X, Wang S, Shu K, Lei T, Zhang H
Stem cell research & therapy 2023 Sep 4;14(1):233

Identification of ferroptosis-related genes in type 2 diabetes mellitus based on machine learning.

Wang S, Lu Y, Chi T, Zhang Y, Zhao Y, Guo H, Feng L
Immunity, inflammation and disease 2023 Oct;11(10):e1036

USP51 facilitates colorectal cancer stemness and chemoresistance by forming a positive feed-forward loop with HIF1A.

Mu M, Zhang Q, Li J, Zhao C, Li X, Chen Z, Sun X, Yu J
Cell death and differentiation 2023 Nov;30(11):2393-2407

rRNA-Derived Small RNA rsRNA-28S Regulates the Chemoresistance of Prostate Cancer Cells by Targeting PTGIS.

Qiao D, Liu Y, Lei Y, Zhang C, Bu Y, Tang Y, Zhang Y
Frontiers in bioscience (Landmark edition) 2023 May 25;28(5):102

Comparative proteomics analysis of transforming growth factor-beta1-overexpressed human dental pulp stem cell-derived secretome on CD44-mediated fibroblast activation via canonical smad signal pathway.

Salkin H, Acar MB, Gonen ZB, Basaran KE, Ozcan S
Connective tissue research 2023 Mar;64(2):205-218

Cdk4 Regulates Glioblastoma Cell Invasion and Stemness and Is Target of a Notch Inhibitor Plus Resveratrol Combined Treatment.

Giordano F, D'Amico M, Montalto FI, Malivindi R, Chimento A, Conforti FL, Pezzi V, Panno ML, Andò S, De Amicis F
International journal of molecular sciences 2023 Jun 13;24(12)

Establishment and validation of a plasma oncofetal chondroitin sulfated proteoglycan for pan-cancer detection.

Zhang PF, Wu ZY, Zhang WB, He YQ, Chen K, Wang TM, Li H, Zheng H, Li DH, Yang DW, Zhou T, Deng CM, Liao Y, Xue WQ, Cao LJ, Li XZ, Zhang JB, Dong SQ, Wang F, Zheng MQ, Zhang WL, Mu J, Jia WH
Nature communications 2023 Feb 6;14(1):645

A histological study of vascular wall resident stem cells in venous malformations.

Xia HF, Lai WQ, Chen GH, Li Y, Xie QH, Jia YL, Chen G, Zhao YF
Cell and tissue research 2022 Nov;390(2):229-243

Bufalin Inhibits Tumorigenesis, Stemness, and Epithelial-Mesenchymal Transition in Colorectal Cancer through a C-Kit/Slug Signaling Axis.

Ding L, Yang Y, Lu Q, Qu D, Chandrakesan P, Feng H, Chen H, Chen X, Liao Z, Du J, Cao Z, Weygant N
International journal of molecular sciences 2022 Nov 1;23(21)

COL11A1-Driven Epithelial-Mesenchymal Transition and Stemness of Pancreatic Cancer Cells Induce Cell Migration and Invasion by Modulating the AKT/GSK-3β/Snail Pathway.

Wang H, Zhou H, Ni H, Shen X
Biomolecules 2022 Mar 2;12(3)

Stress-induced epinephrine promotes epithelial-to-mesenchymal transition and stemness of CRC through the CEBPB/TRIM2/P53 axis.

Zhou Z, Shu Y, Bao H, Han S, Liu Z, Zhao N, Yuan W, Jian C, Shu X
Journal of translational medicine 2022 Jun 7;20(1):262

Glioma-associated mesenchymal stem cells-mediated PD-L1 expression is attenuated by Ad5-Ki67/IL-15 in GBM treatment.

Zhang Q, Zhang J, Wang P, Zhu G, Jin G, Liu F
Stem cell research & therapy 2022 Jun 28;13(1):284

Identification of two cancer stem cell-like populations in triple-negative breast cancer xenografts.

Nakayama J, Matsunaga H, Arikawa K, Yoda T, Hosokawa M, Takeyama H, Yamamoto Y, Semba K
Disease models & mechanisms 2022 Jun 1;15(6)

Exosomal miR-455-3p from BMMSCs prevents cardiac ischemia-reperfusion injury.

Wang Y, Shen Y
Human & experimental toxicology 2022 Jan-Dec;41:9603271221102508

Hyaluronic Acid-Modified Nanoparticles Self-Assembled from Linoleic Acid-Conjugated Chitosan for the Codelivery of miR34a and Doxorubicin in Resistant Breast Cancer.

Yang X, Shang P, Ji J, Malichewe C, Yao Z, Liao J, Du D, Sun C, Wang L, Tang YJ, Guo X
Molecular pharmaceutics 2022 Jan 3;19(1):2-17

Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM).

Laporte MH, Klena N, Hamel V, Guichard P
Nature methods 2022 Feb;19(2):216-222

Afatinib Reverses EMT via Inhibiting CD44-Stat3 Axis to Promote Radiosensitivity in Nasopharyngeal Carcinoma.

Huang H, Huang F, Liang X, Fu Y, Cheng Z, Huang Y, Chen Z, Duan Y, Chen Y
Pharmaceuticals (Basel, Switzerland) 2022 Dec 27;16(1)

Cell transcriptomic atlas of the non-human primate Macaca fascicularis.

Han L, Wei X, Liu C, Volpe G, Zhuang Z, Zou X, Wang Z, Pan T, Yuan Y, Zhang X, Fan P, Guo P, Lai Y, Lei Y, Liu X, Yu F, Shangguan S, Lai G, Deng Q, Liu Y, Wu L, Shi Q, Yu H, Huang Y, Cheng M, Xu J, Liu Y, Wang M, Wang C, Zhang Y, Xie D, Yang Y, Yu Y, Zheng H, Wei Y, Huang F, Lei J, Huang W, Zhu Z, Lu H, Wang B, Wei X, Chen F, Yang T, Du W, Chen J, Xu S, An J, Ward C, Wang Z, Pei Z, Wong CW, Liu X, Zhang H, Liu M, Qin B, Schambach A, Isern J, Feng L, Liu Y, Guo X, Liu Z, Sun Q, Maxwell PH, Barker N, Muñoz-Cánoves P, Gu Y, Mulder J, Uhlen M, Tan T, Liu S, Yang H, Wang J, Hou Y, Xu X, Esteban MA, Liu L
Nature 2022 Apr;604(7907):723-731

Knockdown of ALDH1A3 reduces breast cancer stem cell marker CD44 via the miR-7-TGFBR2-Smad3-CD44 regulatory axis.

Pan M, Li M, Guo M, Zhou H, Xu H, Zhao F, Mei F, Xue R, Dou J
Experimental and therapeutic medicine 2021 Oct;22(4):1093

Bone-targeted erythrocyte-cancer hybrid membrane-camouflaged nanoparticles for enhancing photothermal and hypoxia-activated chemotherapy of bone invasion by OSCC.

Chen H, Deng J, Yao X, He Y, Li H, Jian Z, Tang Y, Zhang X, Zhang J, Dai H
Journal of nanobiotechnology 2021 Oct 26;19(1):342

DOC-2/DAB2 interactive protein destabilizes c-Myc to impair the growth and self-renewal of colon tumor-repopulating cells.

Li H, Zhou Y, Wang M, Wang H, Zhang Y, Peng R, Zhang R, Zhang M, Zhang M, Qiu P, Liu L, Zhao Q, Liu J
Cancer science 2021 Nov;112(11):4593-4603

HOXB7 acts as an oncogenic biomarker in head and neck squamous cell carcinoma.

Wu X, Li J, Yan T, Ke X, Li X, Zhu Y, Yang J, Li Z
Cancer cell international 2021 Jul 24;21(1):393

A Novel Long Non-Coding RNA lnc030 Maintains Breast Cancer Stem Cell Stemness by Stabilizing SQLE mRNA and Increasing Cholesterol Synthesis.

Qin Y, Hou Y, Liu S, Zhu P, Wan X, Zhao M, Peng M, Zeng H, Li Q, Jin T, Cui X, Liu M
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2021 Jan;8(2):2002232

Arl4c promotes the growth and drug resistance of pancreatic cancer by regulating tumor-stromal interactions.

Chen X, Zhang Y, Qian W, Han L, Li W, Duan W, Wu Z, Wang Z, Ma Q
iScience 2021 Dec 17;24(12):103400

Chondrocyte secretome enriched microparticles encapsulated with the chondrocyte membrane to facilitate the chondrogenesis of BMSCs and reduce hypertrophy.

Tang Z, Lu Y, Zhang S, Wang J, Wang Q, Xiao Y, Zhang X
Journal of materials chemistry. B 2021 Dec 15;9(48):9989-10002

Identification of a mesenchymal-related signature associated with clinical prognosis in glioma.

Zhang Z, Chen J, Huo X, Zong G, Huang K, Cheng M, Sun L, Yue X, Bian E, Zhao B
Aging 2021 Apr 19;13(9):12431-12455

Chicken seminal fluid lacks CD9- and CD44-bearing extracellular vesicles.

Alvarez-Rodriguez M, Ntzouni M, Wright D, Khan KI, López-Béjar M, Martinez CA, Rodriguez-Martinez H
Reproduction in domestic animals = Zuchthygiene 2020 Mar;55(3):293-300

Inhibition of DNA Methyltransferase by RG108 Promotes Pluripotency-Related Character of Porcine Bone Marrow Mesenchymal Stem Cells.

Li Q, Zhai Y, Man X, Zhang S, An X
Cellular reprogramming 2020 Apr;22(2):82-89

The Unusual Properties of Polytetrafluoroethylene Enable Massive-Volume Vitrification of Stem Cells with Low-Concentration Cryoprotectants.

Cao Y, Zhao G, Panhwar F, Zhang X, Chen Z, Cheng L, Zang C, Liu F, Zhao Y, He X
Advanced materials technologies 2019 Jan;4(1)

All-Aqueous-Phase Microfluidics for Cell Encapsulation.

Zhu K, Yu Y, Cheng Y, Tian C, Zhao G, Zhao Y
ACS applied materials & interfaces 2019 Feb 6;11(5):4826-4832

Vitrification of stem cell-laden core-shell microfibers with unusually low concentrations of cryoprotective agents.

Tian C, Zhang X, Zhao G
Biomaterials science 2019 Feb 26;7(3):889-900

Exosomes in specific fractions of the boar ejaculate contain CD44: A marker for epididymosomes?

Alvarez-Rodriguez M, Ljunggren SA, Karlsson H, Rodriguez-Martinez H
Theriogenology 2019 Dec;140:143-152

The risk of using monoclonal or polyclonal commercial antibodies: controversial results on porcine sperm CD44 receptor identification.

Alvarez-Rodriguez M, López-Bejar M, Rodriguez-Martinez H
Reproduction in domestic animals = Zuchthygiene 2019 Apr;54(4):733-737

Magnetothermal heating facilitates the cryogenic recovery of stem cell-laden alginate-Fe(3)O(4) nanocomposite hydrogels.

Zhang X, Zhao G, Cao Y, Haider Z, Wang M, Fu J
Biomaterials science 2018 Nov 20;6(12):3139-3151

Hyaluronan improves neither the long-term storage nor the cryosurvival of liquid-stored CD44-bearing AI boar spermatozoa.

Álvarez-Rodriguez M, Vicente-Carrillo A, Rodriguez-Martinez H
The Journal of reproduction and development 2018 Aug 20;64(4):351-360

Bufalin reverses acquired drug resistance by inhibiting stemness in colorectal cancer cells.

Sun J, Xu K, Qiu Y, Gao H, Xu J, Tang Q, Yin P
Oncology reports 2017 Sep;38(3):1420-1430

Hydrogel Encapsulation Facilitates Rapid-Cooling Cryopreservation of Stem Cell-Laden Core-Shell Microcapsules as Cell-Biomaterial Constructs.

Zhao G, Liu X, Zhu K, He X
Advanced healthcare materials 2017 Dec;6(23)

Preliminary identification of endometrial cancer stem cells in vitro and in vivo.

Kong FF, Li D, Yang H, Ma J, Pan X, Liu HX, Huo JN, Ma XX
Biochemical and biophysical research communications 2017 Aug 19;490(2):506-513

Synthesis and characterization of a bimodal nanoparticle based on the host-guest self-assembly for targeted cellular imaging.

Wang H, Sun D, Liao H, Wang Y, Zhao S, Zhang Y, Lv G, Ma X, Liu Y, Sun G
Talanta 2017 Aug 15;171:8-15

GnRH participates in the self-renewal of A549-derived lung cancer stem-like cells through upregulation of the JNK signaling pathway.

Lu C, Huang T, Chen W, Lu H
Oncology reports 2015 Jul;34(1):244-50

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 60224-1-Ig(CD44 antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The CD44 antibody from Proteintech is a mouse monoclonal antibody to a fusion protein of human CD44. This antibody recognizes human antigen. The CD44 antibody has been validated for the following applications: ELISA, WB, IHC, IF analysis.
Appendix: 60224-1-Ig.pdf

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