NBP1-31488

antibody from Novus Biologicals

Targeting: CD44 CD44R, CSPG8, HCELL, IN, MC56, MDU2, MDU3, MIC4, Pgp1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-31488

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-31488, RRID:AB_10003817

Product name

Rabbit Polyclonal CD44 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat, Rabbit

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

DDX3 modulates cisplatin resistance in OSCC through ALKBH5-mediated m⁶A-demethylation of FOXM1 and NANOG.

Shriwas O, Priyadarshini M, Samal SK, Rath R, Panda S, Das Majumdar SK, Muduly DK, Botlagunta M, Dash R
Apoptosis : an international journal on programmed cell death 2020 Apr;25(3-4):233-246

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Loss of tenomodulin results in reduced self-renewal and augmented senescence of tendon stem/progenitor cells.

Alberton P, Dex S, Popov C, Shukunami C, Schieker M, Docheva D
Stem cells and development 2015 Mar 1;24(5):597-609

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Mcph1/Brit1 deficiency promotes genomic instability and tumor formation in a mouse model.

Liang Y, Gao H, Lin SY, Goss JA, Du C, Li K
Oncogene 2015 Aug 13;34(33):4368-78

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CD44 Antibody [NBP1-31488] - A. 30 ug A549 whole cell lysate/extract B. 30 ug H1299 whole cell lysate/extract 7.5 % SDS-PAGE CD44 antibody dilution: 1:5000

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CD44 Antibody [NBP1-31488] - Untreated (-) and treated (+) MDA-MB-231 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody. The observed M.W. is different from the predicted size. It is possibly due to post-translational modifications.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CD44 Antibody [NBP1-31488] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody. The observed M.W. is different from the predicted size. It is possibly due to post-translational modifications.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CD44 Antibody [NBP1-31488] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody diluted at 1:7000. Multiple bands were observed 130-180 kDa. It is possibly due to alternative splicing.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CD44 Antibody [NBP1-31488] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CD44 antibody diluted at 1:5000.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-31488] - Paraffin-embedded Human pancreatic tumor, using antibody at 1:100 dilution.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-31488] - Paraffin-embedded human endometrial carcinoma. CD44 antibody diluted at 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-31488] - Paraffin-embedded Human pancreatic tumor, using antibody at 1:100 dilution.