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LearnM010T02R05
antibody from Invitrogen Antibodies
Targeting: CD44
CD44R, CSPG8, HCELL, IN, MC56, MDU2, MDU3, MIC4, Pgp1
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Flow cytometry [4]
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- Product number
- M010T02R05 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD44 Monoclonal Antibody (IM7), NovaFluor™ Red 725, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1). CD44 is expressed by hematopoietic and non-hematopoietic cells. Bone marrow myeloid cells and memory T cells highly express this antigen and peripheral B and T cells can upregulate the expression of CD44. CD44 functions as an adhesion molecule through its binding to hyaluronate, an extracellular matrix component.
- Antibody clone number
- IM7
- Concentration
- 0.1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- C57BL/6 mouse splenocytes were unstained (left) or stained with 0.6 µg of CD44 Monoclonal Antibody, NovaFluor Red 725 (Product # M010T03R05) (right). All cells were co-stained with CD45R (B220) Monoclonal Antibody, eFluor 450 (Product # 48-0452-82). Total viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD Blue (Product # L34962). Data was acquired on a 5-laser Cytek Aurora and unmixed with autofluorescence extraction.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Spectral signature for NOVAFLUOR RED 725 collected on a 5-laser Cytek Aurora Full Spectrum flow cytometer using Cytek assay settings. Human peripheral blood mononuclear cells were stained with anti-human CD4 (SK3) and signatures displayed following gating on the lymphocyte population.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- C57BL/6 mouse splenocytes were unstained (left) or stained with 0.6 µg of CD44 Monoclonal Antibody, NovaFluor Red 725 (Product # M010T03R05) (right). All cells were co-stained with CD45R (B220) Monoclonal Antibody, eFluor 450 (Product # 48-0452-82). Total viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD Blue (Product # L34962). Data was acquired on a 5-laser Cytek Aurora and unmixed with autofluorescence extraction.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Spectral signature for NOVAFLUOR RED 725 collected on a 5-laser Cytek Aurora Full Spectrum flow cytometer using Cytek assay settings. Human peripheral blood mononuclear cells were stained with anti-human CD4 (SK3) and signatures displayed following gating on the lymphocyte population.
