NB500-481

antibody from Novus Biologicals

Targeting: CD44 CD44R, CSPG8, HCELL, IN, MC56, MDU2, MDU3, MIC4, Pgp1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB500-481

Provider

Novus Biologicals

Proper citation

Novus Cat#NB500-481, RRID:AB_10000604

Product name

Mouse Monoclonal CD44 Antibody

Antibody type

Monoclonal

Description

Protein A purified. The antibody MEM-263 reacts with extracellular (N-terminal) domain of standard CD44 (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.)

Reactivity

Human, Canine, Porcine

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

Metformin reveals a mitochondrial copper addiction of mesenchymal cancer cells.

Müller S, Versini A, Sindikubwabo F, Belthier G, Niyomchon S, Pannequin J, Grimaud L, Cañeque T, Rodriguez R
PloS one 2018;13(11):e0206764

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Hyaluronic acid effect on adipose-derived stem cells. Biological in vitro evaluation.

Moreno A, Martínez A, Olmedillas S, Bello S, de Miguel F
Revista espanola de cirugia ortopedica y traumatologia 2015 Jul-Aug;59(4):215-21

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Biopsy-induced inflammatory conditions improve endometrial receptivity: the mechanism of action.

Gnainsky Y, Granot I, Aldo P, Barash A, Or Y, Mor G, Dekel N
Reproduction (Cambridge, England) 2015 Jan;149(1):75-85

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A translational approach in using cell sheet fragments of autologous bone marrow-derived mesenchymal stem cells for cellular cardiomyoplasty in a porcine model.

Huang CC, Tsai HW, Lee WY, Lin WW, Chen DY, Hung YW, Chen JW, Hwang SM, Chang Y, Sung HW
Biomaterials 2013 Jun;34(19):4582-91

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Prevalence of epithelial ovarian cancer stem cells correlates with recurrence in early-stage ovarian cancer.

Steffensen KD, Alvero AB, Yang Y, Waldstrøm M, Hui P, Holmberg JC, Silasi DA, Jakobsen A, Rutherford T, Mor G
Journal of oncology 2011;2011:620523

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CD44 Antibody (MEM-263) [NB500-481] - Fig. 1. Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-CD44 (MEM-263). Lane 1: lysate of human PBL Lane 2: lysate of canine PBL Lane 3: lysate of porcine PBL.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CD44 Antibody (MEM-263) [NB500-481] - Analysis of CD44 in MOLT-4 cells (lane 1) and HeLa cells (lane 2) using anti-CD44 (MEM-263) purified; non-reducing conditions.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CD44 Antibody (MEM-263) [NB500-481] - Expression of CD44 and CK18 in multiple clones of ovarian cancer cells. CD44+ cells also express CK18 as determined by western blot analysis. Image collected and cropped by CiteAb from the following publication (http://www.hindawi.com/journals/jo/2011/620523/) licensed under a CC-BY licence.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD44 Antibody (MEM-263) [NB500-481] - A surface stain was performed on hPBMCs with CD44 (MEM-263) antibody NB500-481AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD44 Antibody (MEM-263) [NB500-481] - Analysis of human peripheral blood (lymphocyte gate) using anti-CD44 (MEM-263) PE conjugate.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD44 Antibody (MEM-263) [NB500-481] - Staining of human peripheral blood using anti-CD44 (MEM-263) FITC conjugate.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD44 Antibody (MEM-263) [NB500-481] - Staining of human peripheral blood using anti-CD44 (MEM-263) APC conjugate.