NBP1-47386
antibody from Novus Biologicals
Targeting: CD44
CD44R, CSPG8, HCELL, IN, MC56, MDU2, MDU3, MIC4, Pgp1
Antibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [2]
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Validation data
Reference
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- Product number
- NBP1-47386 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-47386, RRID:AB_10010339
- Product name
- Mouse Monoclonal CD44 Antibody
- Antibody type
- Monoclonal
- Description
- Ammonium sulfate precipitation.
- Reactivity
- Human, Mouse, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Smooth Muscle Cell Reprogramming in Aortic Aneurysms.
Novel K6-K14 keratin fusion enhances cancer stemness and aggressiveness in oral squamous cell carcinoma.
Breast osteoblast-like cells: a new biomarker for the management of breast cancer.
Epidermal-specific deletion of TC-PTP promotes UVB-induced epidermal cell survival through the regulation of Flk-1/JNK signaling.
Association between inflammation and cancer stem cell phenotype in breast cancer.
Chemotherapy induces enrichment of CD47+/CD73+/PDL1+ immune evasive triple-negative breast cancer cells.
Using paracrine effects of Ad-MSCs on keratinocyte cultivation and fabrication of epidermal sheets for improving clinical applications.
Drug-resistant colon cancer cells produce high carcinoembryonic antigen and might not be cancer-initiating cells.
Tumor-derived macrophage migration inhibitory factor promotes an autocrine loop that enhances renal cell carcinoma.
TLR2 enhances ovarian cancer stem cell self-renewal and promotes tumor repair and recurrence.
Steroids and β2-agonists regulate hyaluronan metabolism in asthmatic airway smooth muscle cells.
Chen PY, Qin L, Li G, Malagon-Lopez J, Wang Z, Bergaya S, Gujja S, Caulk AW, Murtada SI, Zhang X, Zhuang ZW, Rao DA, Wang G, Tobiasova Z, Jiang B, Montgomery RR, Sun L, Sun H, Fisher EA, Gulcher JR, Fernandez-Hernando C, Humphrey JD, Tellides G, Chittenden TW, Simons M
Cell stem cell 2020 Apr 2;26(4):542-557.e11
Cell stem cell 2020 Apr 2;26(4):542-557.e11
Novel K6-K14 keratin fusion enhances cancer stemness and aggressiveness in oral squamous cell carcinoma.
Tsai FJ, Lai MT, Cheng J, Chao SC, Korla PK, Chen HJ, Lin CM, Tsai MH, Hua CH, Jan CI, Jinawath N, Wu CC, Chen CM, Kuo BY, Chen LW, Yang J, Hwang T, Sheu JJ
Oncogene 2019 Jun;38(26):5113-5126
Oncogene 2019 Jun;38(26):5113-5126
Breast osteoblast-like cells: a new biomarker for the management of breast cancer.
Scimeca M, Urbano N, Bonfiglio R, Schillaci O, Bonanno E
British journal of cancer 2018 Oct;119(9):1129-1132
British journal of cancer 2018 Oct;119(9):1129-1132
Epidermal-specific deletion of TC-PTP promotes UVB-induced epidermal cell survival through the regulation of Flk-1/JNK signaling.
Baek M, Kim M, Lim JS, Morales LD, Hernandez J, Mummidi S, Williams-Blangero S, Jang IS, Tsin AT, Kim DJ
Cell death & disease 2018 Jun 28;9(7):730
Cell death & disease 2018 Jun 28;9(7):730
Association between inflammation and cancer stem cell phenotype in breast cancer.
Jeong YJ, Oh HK, Park SH, Bong JG
Oncology letters 2018 Feb;15(2):2380-2386
Oncology letters 2018 Feb;15(2):2380-2386
Chemotherapy induces enrichment of CD47+/CD73+/PDL1+ immune evasive triple-negative breast cancer cells.
Samanta D, Park Y, Ni X, Li H, Zahnow CA, Gabrielson E, Pan F, Semenza GL
Proceedings of the National Academy of Sciences of the United States of America 2018 Feb 6;115(6):E1239-E1248
Proceedings of the National Academy of Sciences of the United States of America 2018 Feb 6;115(6):E1239-E1248
Using paracrine effects of Ad-MSCs on keratinocyte cultivation and fabrication of epidermal sheets for improving clinical applications.
Hassanzadeh H, Matin MM, Naderi-Meshkin H, Bidkhori HR, Mirahmadi M, Raeesolmohaddeseen M, Sanjar-Moussavi N, Bahrami AR
Cell and tissue banking 2018 Dec;19(4):531-547
Cell and tissue banking 2018 Dec;19(4):531-547
Drug-resistant colon cancer cells produce high carcinoembryonic antigen and might not be cancer-initiating cells.
Lee HC, Ling QD, Yu WC, Hung CM, Kao TC, Huang YW, Higuchi A
Drug design, development and therapy 2013;7:491-502
Drug design, development and therapy 2013;7:491-502
Tumor-derived macrophage migration inhibitory factor promotes an autocrine loop that enhances renal cell carcinoma.
Du W, Wright BM, Li X, Finke J, Rini BI, Zhou M, He H, Lal P, Welford SM
Oncogene 2013 Mar 14;32(11):1469-74
Oncogene 2013 Mar 14;32(11):1469-74
TLR2 enhances ovarian cancer stem cell self-renewal and promotes tumor repair and recurrence.
Chefetz I, Alvero AB, Holmberg JC, Lebowitz N, Craveiro V, Yang-Hartwich Y, Yin G, Squillace L, Gurrea Soteras M, Aldo P, Mor G
Cell cycle (Georgetown, Tex.) 2013 Feb 1;12(3):511-21
Cell cycle (Georgetown, Tex.) 2013 Feb 1;12(3):511-21
Steroids and β2-agonists regulate hyaluronan metabolism in asthmatic airway smooth muscle cells.
Papakonstantinou E, Klagas I, Karakiulakis G, Hostettler K, S'ng CT, Kotoula V, Savic S, Tamm M, Roth M
American journal of respiratory cell and molecular biology 2012 Dec;47(6):759-67
American journal of respiratory cell and molecular biology 2012 Dec;47(6):759-67
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis using anti-CD44 mAb against HeLa (1) and HUVE-12 (2) cell lysates.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis using anti-CD44 mAb against truncated Trx-CD44 recombinant protein (1) and GST-CD44 (aa628-699) recombinant protein (2).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of FFPE human breast carcinoma tissues using CD44 antibody (8E2F3). The signal was developed using DAB based detection and the sections were processed for counterstaining with hematoxylin. The antibody generated mainly a membrane staining representative of CD44 protein.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of HeLa cells using anti-CD44 mAb (right) and negative control (left).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD44 Antibody (8E2F3) [NBP1-47386] - An intracellular stain was performed on HeLa cells with NBP1-47386AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.