NBP1-84578
antibody from Novus Biologicals
Targeting: CD44
CD44R, CSPG8, HCELL, IN, MC56, MDU2, MDU3, MIC4, Pgp1
Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [6]
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Validation data
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- Product number
- NBP1-84578 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-84578, RRID:AB_11033613
- Product name
- Rabbit Polyclonal CD44 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human CD44 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Correction: EpCAM-Independent Enrichment of Circulating Tumor Cells in Metastatic Breast Cancer.
Superficial scrapings from breast tumors is a source for biobanking and research purposes.
Optimization of tumor xenograft dissociation for the profiling of cell surface markers and nutrient transporters.
Identification of a population of blood circulating tumor cells from breast cancer patients that initiates metastasis in a xenograft assay.
Metformin regulates breast cancer stem cell ontogeny by transcriptional regulation of the epithelial-mesenchymal transition (EMT) status.
Synthesis of hyaluronan in oesophageal cancer cells is uncoupled from the prostaglandin-cAMP pathway.
Expression profiling of microdissected cell populations selected from basal cells in normal epidermis and basal cell carcinoma.
Schneck H, Gierke B, Uppenkamp F, Behrens B, Niederacher D, Stoecklein NH, Templin MF, Pawlak M, Fehm T, Neubauer H, Disseminated Cancer Cell Network (DCC Net) Duesseldorf.
PloS one 2016;11(2):e0149315
PloS one 2016;11(2):e0149315
Superficial scrapings from breast tumors is a source for biobanking and research purposes.
Ma R, Fredriksson I, Karthik GM, Winn G, Darai-Ramqvist E, Bergh J, Hartman J
Laboratory investigation; a journal of technical methods and pathology 2014 Jul;94(7):796-805
Laboratory investigation; a journal of technical methods and pathology 2014 Jul;94(7):796-805
Optimization of tumor xenograft dissociation for the profiling of cell surface markers and nutrient transporters.
Petit V, Massonnet G, Maciorowski Z, Touhami J, Thuleau A, Némati F, Laval J, Château-Joubert S, Servely JL, Vallerand D, Fontaine JJ, Taylor N, Battini JL, Sitbon M, Decaudin D
Laboratory investigation; a journal of technical methods and pathology 2013 May;93(5):611-21
Laboratory investigation; a journal of technical methods and pathology 2013 May;93(5):611-21
Identification of a population of blood circulating tumor cells from breast cancer patients that initiates metastasis in a xenograft assay.
Baccelli I, Schneeweiss A, Riethdorf S, Stenzinger A, Schillert A, Vogel V, Klein C, Saini M, Bäuerle T, Wallwiener M, Holland-Letz T, Höfner T, Sprick M, Scharpff M, Marmé F, Sinn HP, Pantel K, Weichert W, Trumpp A
Nature biotechnology 2013 Jun;31(6):539-44
Nature biotechnology 2013 Jun;31(6):539-44
Metformin regulates breast cancer stem cell ontogeny by transcriptional regulation of the epithelial-mesenchymal transition (EMT) status.
Vazquez-Martin A, Oliveras-Ferraros C, Cufí S, Del Barco S, Martin-Castillo B, Menendez JA
Cell cycle (Georgetown, Tex.) 2010 Sep 15;9(18):3807-14
Cell cycle (Georgetown, Tex.) 2010 Sep 15;9(18):3807-14
Synthesis of hyaluronan in oesophageal cancer cells is uncoupled from the prostaglandin-cAMP pathway.
Twarock S, Röck K, Sarbia M, Weber AA, Jänicke RU, Fischer JW
British journal of pharmacology 2009 May;157(2):234-43
British journal of pharmacology 2009 May;157(2):234-43
Expression profiling of microdissected cell populations selected from basal cells in normal epidermis and basal cell carcinoma.
Asplund A, Gry Björklund M, Sundquist C, Strömberg S, Edlund K, Ostman A, Nilsson P, Pontén F, Lundeberg J
The British journal of dermatology 2008 Mar;158(3):527-38
The British journal of dermatology 2008 Mar;158(3):527-38
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD44 Antibody [NBP1-84578] - Analysis in human cell lines U-251MG and MCF-7. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Staining of human squamous cell lung carcinoma shows strong membranous positivity in tumor cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Staining of human skin shows strong membranous positivity in squamous epithelial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Staining of human breast shows strong membranous positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Staining in human skin and skeletal muscle tissues . Corresponding CD44 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Staining of human lymph node shows strong positivity in plasma membrane in non-germinal center cells.