M010T02R04-A

antibody from Invitrogen Antibodies
Targeting: CD44 CD44R, CSPG8, HCELL, IN, MC56, MDU2, MDU3, MIC4, Pgp1
Provider product page for M010T02R04-A

Antibody data

Product number
M010T02R04-A - Provider product page
Provider
Invitrogen Antibodies
Product name
CD44 Monoclonal Antibody (IM7), NovaFluor™ Red 710, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description<br>The IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1). CD44 is expressed by hematopoietic and non-hematopoietic cells. Bone marrow myeloid cells and memory T cells highly express this antigen and peripheral B and T cells can upregulate the expression of CD44. CD44 functions as an adhesion molecule through its binding to hyaluronate, an extracellular matrix component.<br><br>This product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer.<br><br>Applications Tested<br>The IM7 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocytes. This can be used at less than or equal to 0.6 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.<br><br>Master mixes<br>• Master mixes of NFs should be made at 2-8 °C and may be made up to 4 hours ahead of time.<br>• We do not recommend storing master mixes containing NovaFluor conjugates overnight or longer.<br><br>Whole Blood compatibility<br>• When utilizing whole blood (as opposed to density-gradient-purified PBMC), we recommend lysing red blood cells in bulk prior to staining with NovaFluor conjugates.<br>• See the Bulk Lysis of Human Whole Blood protocol <a href="https://www.thermofisher.com/us/en/home/references/protocols/cell-and-tissue-analysis/protocols/red-blood-cell-lysis.html">here</a>.<br>• Staining of whole blood with NovaFluor conjugates followed by lysis of red blood cells may result in higher-than-expected background staining.<br><br>Viability dye compatibility<br>• NovaFluor dyes are not compatible with DNA intercalating viability dyes.<br>• Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. <a href="https://www.thermofisher.com/us/en/home/life-science/cell-analysis/flow-cytometry/flow-cytometry-assays-reagents/cell-viability-assays-flow-cytometry/fixable-viability-dyes-flow-cytometry.html">Invitrogen LIVE/DEAD Fixable Dead Cell stains</a> are recommended for use with NovaFluor dyes.<br><br>CellBlox Plus Blocking Buffer<br>• This NovaFluor conjugate comes with CellBlox Plus Blocking Buffer (Cat. No. C001T03F01), essential for optimal staining.<br>• Use CellBlox Plus Blocking Buffer in all experiments with NovaFluor conjugates.<br>• Add 5 µL per sample to antibody cocktails/master mixes (regardless of how many Novafluor-conjugated antibodies are present) before combining with cells.<br>• CellBlox Plus Blocking Buffer is compatible with either Super Bright Complete Blocking Buffer or Brilliant Stain Buffer and can be used in antibody cocktails/master mixes with those reagents.<br>• For single-color controls, use 5 µL of CellBlox Plus Blocking Buffer per 100 µL of cell sample (10^3 to 10^8 cells).<br><br>NovaFluor conjugates are based on Phiton technology utilizing novel fluorophore-containing nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. <a href="https://www.thermofisher.com/us/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/novafluor-dyes.html">Learn more</a><br><br>Excitation: 637 nm; Emission: 713 nm; Laser: 633-640 nm (Red) Laser
Reactivity
Human, Mouse
Host
Rat
Isotype
IgG
Antibody clone number
IM7
Vial size
25 μg
Concentration
0.1 mg/mL
Storage
4°C, store in dark, DO NOT FREEZE!
CD44 protein structure - M010T02R04-A shown in red.