- PA5-21419 - Invitrogen Antibodies CD44 antibody

Submitted references The vascular bone marrow niche influences outcome in chronic myeloid leukemia via the E-selectin - SCL/TAL1 - CD44 axis.

Godavarthy PS, Kumar R, Herkt SC, Pereira RS, Hayduk N, Weissenberger ES, Aggoune D, Manavski Y, Lucas T, Pan KT, Voutsinas JM, Wu Q, Müller MC, Saussele S, Oellerich T, Oehler VG, Lausen J, Krause DS
Haematologica 2020 Jan;105(1):136-147

The absence of aldehyde dehydrogenase 1 A1-positive cells in benign mammary stroma is associated with risk factors for breast cancer.

Isfoss BL, Holmqvist B, Jernström H, Alm P, Olsson H
Breast cancer (Dove Medical Press) 2016;8:117-24

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CD44/H-CAM using Whole cell extracts (30 µg). Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a CD44/H-CAM polyclonal antibody (Product # PA5-21419) at a dilution of 1:5000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CD44/H-CAM using A) 30 µg A549 whole cell lysate and B) 30 µg H1299 whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a CD44/H-CAM polyclonal antibody (Product # PA5-21419) at a dilution of 1:5000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of CD44 was performed by separating 30 µg of untreated (–) and treated (+) MDA-MB-231 whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a CD44 Polyclonal Antibody (Product # PA5-21419) at a dilution of 1:7000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of CD44 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a CD44 Polyclonal Antibody (Product # PA5-21419) at a dilution of 1:7000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using CD44 Polyclonal Antibody (Product # PA5-21419). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD44 Polyclonal Antibody (Product # PA5-21419) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of CD44/H-CAM in methanol-fixed HeLa cells using a CD44/H-CAM polyclonal antibody (Product # PA5-21419) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using CD44 antibody (Product # PA5-21419) (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Product # MA1-25054) (Red) at 1:2,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: CD44 Polyclonal Antibody detects CD44 protein at cell membrane and cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: CD44 stained by CD44 Polyclonal Antibody (Product # PA5-21419) diluted at 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: CD44 Polyclonal Antibody detects CD44 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. CD44 stained by CD44 Polyclonal Antibody (Product # PA5-21419) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: CD44 Polyclonal Antibody detects CD44 protein at membrane and cytoplasm on human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. CD44 Polyclonal Antibody (Product # PA5-21419) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded Human pancreatic tumor, using CD44 (Product # PA5-21419) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.