GTX628895

antibody from GeneTex

Targeting: CD44 CD44R, CSPG8, HCELL, IN, MC56, MDU2, MDU3, MIC4, Pgp1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX628895

Provider

GeneTex

Product name

CD44 antibody [GT462]

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

References

Submitted references

A high-throughput pipeline for validation of antibodies.

Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912

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Functional Tissue Engineering: A Prevascularized Cardiac Muscle Construct for Validating Human Mesenchymal Stem Cells Engraftment Potential In Vitro.

Valarmathi MT, Fuseler JW, Potts JD, Davis JM, Price RL
Tissue engineering. Part A 2018 Jan;24(1-2):157-185

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Hypoxia promotes acquisition of aggressive phenotypes in human malignant mesothelioma.

Kim MC, Hwang SH, Kim NY, Lee HS, Ji S, Yang Y, Kim Y
BMC cancer 2018 Aug 15;18(1):819

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

CD44 antibody [GT462] detects CD44 protein by Western blot analysis.A. 30 ?g H1299 whole cell lysate/extract7.5 % SDS-PAGECD44 antibody [GT462] (GTX628895) dilution: 1:3000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Untreated (¡V) and treated (+) MDA-MB-231 whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody [GT462] (GTX628895) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Wild-type (WT) and CD44 knockout (KO) HeLa cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody [GT462] (GTX628895) diluted at 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Untreated (¡V) and treated (+) MDA-MB-231 whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody [GT462] (GTX628895) diluted at 1:1000.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody [GT462] (GTX628895) diluted at 1:1000.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Wild-type (WT) and CD44 knockout (KO) HeLa cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody [GT462] (GTX628895) diluted at 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Untreated (¡V) and treated (+) HeLa whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody [GT462] (GTX628895) diluted at 1:1000.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

CD44 antibody [GT462] detects CD44 protein at membrane on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. CD44 antibody [GT462] (GTX628895) dilution: 1:200.

Flow cytometry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

CD44 antibody detects CD44 protein by flow cytometry analysis.Sample: HL-60 cell fixed in 4% paraformaldehyde at 4?C for 5 min.Black: Isotype control dilution: 1:100.Green: CD44 antibody (GTX628895) dilution: 1:100.