Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- 49-1034 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RFXAP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- ChIP assays were performed using NALM cells (a cell line derived from human pre-B leukemia), the anti-RFXAP antibody (Product # 49-1034), and optimized primer sets. Each ChIP assay used sheared chromatin from 2 million cells and 5 µg (lane 3) and 7 µg (lane 4) of anti-RFXAP antibody. An anti-flag antibody (lane 2) is used as negative IP control. The HLA_DRA primer pair was used as positive PCR control, and HLA_DOB was used a negative PCR control. The ChIP results using the anti-RFXAP antibody (Product # 49-1034) show a clear signal for the positive primer pair (HLA_DRA) with respectively 5 and 7 µg of antibody per IP (lower panel, lanes 3 and 4) and show no signal for the negative primer pair (HLA_DOB) (upper panel, lanes 3 and 4). The input is shown in lane 1 and the negative IP control (anti-flag antibody) is shown in lane 2.