Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 16296-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#16296-1-AP, RRID:AB_2111275
- Product name
- GHITM antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated GHITM antibody (Cat. #16296-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, WB,ELISA.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Chronic cold exposure induces plasticity of mitochondrial calcium uptake in beige and brown fat of UCP1-deficient mice.
Interaction of the mitochondrial calcium/proton exchanger TMBIM5 with MICU1.
Impact of PARL-mediated mitochondrial protease activity on calcium regulation.
Mitochondrial calcium uniporter complex controls T-cell-mediated immune responses.
Varicella zoster virus glycoprotein E facilitates PINK1/Parkin-mediated mitophagy to evade STING and MAVS-mediated antiviral innate immunity.
Cyclophilin D plays a critical role in the survival of senescent cells.
GHITM regulates malignant phenotype and sensitivity to PD-1 blockade of renal cancer cells via Notch signalling.
TMBIM5 loss of function alters mitochondrial matrix ion homeostasis and causes a skeletal myopathy.
Chaperone-mediated autophagy degrades Keap1 and promotes Nrf2-mediated antioxidative response.
Early macrophage response to obesity encompasses Interferon Regulatory Factor 5 regulated mitochondrial architecture remodelling.
Regulation of mitochondrial proteostasis by the proton gradient.
Transmembrane BAX Inhibitor-1 Motif Containing Protein 5 (TMBIM5) Sustains Mitochondrial Structure, Shape, and Function by Impacting the Mitochondrial Protein Synthesis Machinery.
Chamorro CG, Pathuri S, Acin-Perez R, Chhan M, Milner MG, Ermolova N, Jones AE, Divakaruni AS, Stiles L, Hevener AL, Zhou Z, Shirihai OS, Kirichok Y, Bertholet AM
bioRxiv : the preprint server for biology 2026 Mar 18;
bioRxiv : the preprint server for biology 2026 Mar 18;
Interaction of the mitochondrial calcium/proton exchanger TMBIM5 with MICU1.
Zhang L, Gottschalk B, Dietsche F, Bitar S, Bueno D, Rojas-Charry L, Kumari A, Garg V, Graier WF, Methner A
Communications biology 2025 Sep 19;8(1):1348
Communications biology 2025 Sep 19;8(1):1348
Impact of PARL-mediated mitochondrial protease activity on calcium regulation.
D'Angelo D, Al Saidi A, Ghirardo G, Vecellio Reane D, Gasparini N, Rizzuto R, Raffaello A
Biochimica et biophysica acta. Molecular cell research 2025 Oct;1872(7):119998
Biochimica et biophysica acta. Molecular cell research 2025 Oct;1872(7):119998
Mitochondrial calcium uniporter complex controls T-cell-mediated immune responses.
Shumanska M, Lodygin D, Gibhardt CS, Ickes C, Stejerean-Todoran I, Krause LCM, Pahl K, Jacobs LJHC, Paluschkiwitz A, Liu S, Boshnakovska A, Voigt N, Legler TJ, Haubrock M, Mitkovski M, Poschmann G, Rehling P, Dennerlein S, Riemer J, Flügel A, Bogeski I
EMBO reports 2025 Jan;26(2):407-442
EMBO reports 2025 Jan;26(2):407-442
Varicella zoster virus glycoprotein E facilitates PINK1/Parkin-mediated mitophagy to evade STING and MAVS-mediated antiviral innate immunity.
Oh SJ, Yu JW, Ahn JH, Choi ST, Park H, Yun J, Shin OS
Cell death & disease 2024 Jan 6;15(1):16
Cell death & disease 2024 Jan 6;15(1):16
Cyclophilin D plays a critical role in the survival of senescent cells.
Protasoni M, López-Polo V, Stephan-Otto Attolini C, Brandariz J, Herranz N, Mateo J, Ruiz S, Fernandez-Capetillo O, Kovatcheva M, Serrano M
The EMBO journal 2024 Dec;43(23):5972-6000
The EMBO journal 2024 Dec;43(23):5972-6000
GHITM regulates malignant phenotype and sensitivity to PD-1 blockade of renal cancer cells via Notch signalling.
Huang S, Liu J, Hu J, Hou Y, Hu M, Zhang B, Luo H, Fu S, Chen Y, Liu X, Chen Z, Wang L
Journal of cellular and molecular medicine 2024 Apr;28(8):e18290
Journal of cellular and molecular medicine 2024 Apr;28(8):e18290
TMBIM5 loss of function alters mitochondrial matrix ion homeostasis and causes a skeletal myopathy.
Zhang L, Dietsche F, Seitaj B, Rojas-Charry L, Latchman N, Tomar D, Wüst RC, Nickel A, Frauenknecht KB, Schoser B, Schumann S, Schmeisser MJ, Vom Berg J, Buch T, Finger S, Wenzel P, Maack C, Elrod JW, Parys JB, Bultynck G, Methner A
Life science alliance 2022 Oct;5(10)
Life science alliance 2022 Oct;5(10)
Chaperone-mediated autophagy degrades Keap1 and promotes Nrf2-mediated antioxidative response.
Zhu L, He S, Huang L, Ren D, Nie T, Tao K, Xia L, Lu F, Mao Z, Yang Q
Aging cell 2022 Jun;21(6):e13616
Aging cell 2022 Jun;21(6):e13616
Early macrophage response to obesity encompasses Interferon Regulatory Factor 5 regulated mitochondrial architecture remodelling.
Orliaguet L, Ejlalmanesh T, Humbert A, Ballaire R, Diedisheim M, Julla JB, Chokr D, Cuenco J, Michieletto J, Charbit J, Lindén D, Boucher J, Potier C, Hamimi A, Lemoine S, Blugeon C, Legoix P, Lameiras S, Baudrin LG, Baulande S, Soprani A, Castelli FA, Fenaille F, Riveline JP, Dalmas E, Rieusset J, Gautier JF, Venteclef N, Alzaid F
Nature communications 2022 Aug 30;13(1):5089
Nature communications 2022 Aug 30;13(1):5089
Regulation of mitochondrial proteostasis by the proton gradient.
Patron M, Tarasenko D, Nolte H, Kroczek L, Ghosh M, Ohba Y, Lasarzewski Y, Ahmadi ZA, Cabrera-Orefice A, Eyiama A, Kellermann T, Rugarli EI, Brandt U, Meinecke M, Langer T
The EMBO journal 2022 Aug 16;41(16):e110476
The EMBO journal 2022 Aug 16;41(16):e110476
Transmembrane BAX Inhibitor-1 Motif Containing Protein 5 (TMBIM5) Sustains Mitochondrial Structure, Shape, and Function by Impacting the Mitochondrial Protein Synthesis Machinery.
Seitaj B, Maull F, Zhang L, Wüllner V, Wolf C, Schippers P, La Rovere R, Distler U, Tenzer S, Parys JB, Bultynck G, Methner A
Cells 2020 Sep 23;9(10)
Cells 2020 Sep 23;9(10)
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 16296-1-AP(GHITM antibody) at dilution of 1:400
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human brain using 16296-1-AP(GHITM antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The GHITM antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human GHITM. This antibody recognizes human antigen. The GHITM antibody has been validated for the following applications: ELISA, WB, IHC analysis.
