Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunohistochemistry [2]
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- Product number
- 41-9791-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRAF-1 Monoclonal Antibody (1F3), eFluor™ 570, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody 1F3 recognizes human TRAF-1. TRAF-1 is a multifunctional adapter molecule and member of the TNF receptor associated factor protein family. TRAF proteins associate with various receptors of the TNF receptor superfamily to mediate signal transduction. TRAF-1 plays a role in regulating cell survival and apoptosis, regulates the signaling of NF kappa B and JNK, and forms a heterodimeric complex with TRAF-2. TRAF-1 also interacts with cell surface receptors such as CD30, CD40, and CD357 (AITR), and cytoplasmic proteins like NIK, RIP2, A20, and TRIP. Expression of TRAF-1 can be induced by Epstein-Barr Virus (EBV) and TNF alpha. Applications Reported: This 1F3 antibody has been reported for use in microscopy, immunohistochemical staining, and immunocytochemistry. Applications Tested: This 1F3 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable. Excitation: 555 nm; Emission: 570 nm
- Reactivity
- Human
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- 1F3
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of formalin-fixed paraffin embedded human tonsil tissue using 5 µg/mL of Rat IgG2a K Isotype Control eFluor® 570 (left) or 5 µg/mL of Anti-Human TRAF-1 eFluor® 570 (right). Nuclei are stained with DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of formalin-fixed paraffin embedded human tonsil tissue using 5 µg/mL of Rat IgG2a K Isotype Control eFluor® 570 (left) or 5 µg/mL of Anti-Human TRAF-1 eFluor® 570 (right). Nuclei are stained with DAPI.