Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF3276 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TRAF-1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human TRAF-1 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human TRAF-2, -3, -4, -5, and -6 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q13077
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human TRAF-1 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human TRAF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3276) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional refer-ence, recombinant human TRAF-1, TRAF-2, TRAF-3, TRAF-4, TRAF-5, and TRAF-6 (2 ng/lane) were included. A specific band for TRAF-1 was detected at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.