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Western blot
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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunoprecipitation [3]
- Immunohistochemistry [1]
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- Product number
- LS-B11438 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ ISG15 Antibody LS-B11438
- Antibody type
- Polyclonal
- Description
- Caprylic acid and ammonium sulfate precipitation
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of Ubiquitin-like protein ISG15 antibody at 2 ug/ml + 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 18 kDa. Observed band size: 18 kDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot All lanes: Ubiquitin-like protein ISG15 antibody at 2µg/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 18 kDa Observed band size: 18 kDa
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitating ISG15 in Hela whole cell lysate Lane 1: Rabbit control IgG instead of ISG15 Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/5000) Lane 2: ISG15 Antibody (8µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (20µg)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating ISG15 in Hela whole cell lysate Lane 1: Rabbit control IgG instead of ISG15 Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/5000) Lane 2: ISG15 Antibody (8µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (20µg)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating ISG15 in Hela whole cell lysate Lane 1: Rabbit control IgG instead of ISG15 Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/5000) Lane 2: ISG15 Antibody (8µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (20µg)
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-ISG15 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
