MAB4845

antibody from Novus Biologicals

Targeting: ISG15 G1P2, IFI15, UCRP

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

MAB4845

Provider

Novus Biologicals

Product name

Mouse Monoclonal ISG15/UCRP Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human ISG15/UCRP in direct ELISAs and Western blots.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Vitamin D receptor and Jak-STAT signaling crosstalk results in calcitriol-mediated increase of hepatocellular response to IFN-α.

Lange CM, Gouttenoire J, Duong FH, Morikawa K, Heim MH, Moradpour D
Journal of immunology (Baltimore, Md. : 1950) 2014 Jun 15;192(12):6037-44

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human ISG15/UCRP by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human ISG15/UCRP Monoclonal Antibody (Catalog # MAB4845) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ISG15/UCRP at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.