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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
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- Product number
- IC8044P - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal ISG15/UCRP Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human ISG15/UCRP in ELISAs and Western Blot.
- Reactivity
- Human
- Host
- Rat
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Submitted references Reshaping of the Dendritic Cell Chromatin Landscape and Interferon Pathways during HIV Infection.
Johnson JS, Lucas SY, Amon LM, Skelton S, Nazitto R, Carbonetti S, Sather DN, Littman DR, Aderem A
Cell host & microbe 2018 Mar 14;23(3):366-381.e9
Cell host & microbe 2018 Mar 14;23(3):366-381.e9
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of ISG15/UCRP in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line untreated (light orange filled histogram) or treated with 500 U/mL Recombinant Human IFN-beta 1a (Mammalian) (Catalog # 11415-1) for 16 hours (dark orange filled histogram) was stained with Rat Anti-Human ISG15/UCRP PE-conjugated Monoclonal Antibody (Catalog # IC8044P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
