Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- ARP59386_P050 - Provider product page
- Provider
- Aviva Systems Biology
- Proper citation
- Aviva Systems Biology Cat#ARP59386_P050, RRID:AB_10886598
- Product name
- ISG15 antibody - middle region (ARP59386_P050)
- Antibody type
- Polyclonal
- Antigen
- The immunogen is a synthetic peptide directed towards the middle region of human ISG15
- Description
- Affinity Purified
- Reactivity
- Human, Bovine, Canine, Goat, Horse, Porcine, Rabbit, Sheep
- Host
- Rabbit
- Vial size
- 100 μl
- Concentration
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- Storage
- For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Sample Type : 1. Human NT-2 cells (60ug) 2. mouse brain extracts (80ug) Primary Antibody Dilution: 2ug/ml, Secondary Antibody : IRDye 800CW goat anti-rabbit from Li-COR Bioscience Secondary Antibody Dilution: 1: 20,000 Image Submitted by : Yuzhi Chen University of Arkansas for Medical Science
- Sample type
- NT-2 cell line, mouse brain
- Primary Ab dilution
- 0.2-1 µg/mL
- Protocol
- Protocol
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- WB Suggested Anti-ISG15 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:1562500, Positive Control: Human kidney
- Sample type
- NT-2 cell line, mouse brain
- Primary Ab dilution
- 2 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Researcher: Dr. Yuzhi Chen, University of Arkansas for Medical ScienceApplication: IHCSpecies+tissue/cell type: Mouse brain stem cellsPrimary antibody dilution: 1:500Secondary antibody: Goat anti-rabbit Alexa-Fluor 594Secondary antibody dilution: 1:1000