Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- A-830 - Provider product page
- Provider
- R&D Systems
- Product name
- Human ISG15/UCRP Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. This antibody will recover ISGylated proteins in immunoprecipitation applications. This antibody detects ISGylated proteins in Western blots.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P05161
- Isotype
- IgG
- Antibody clone number
- 851701
- Vial size
- 50 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C, as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 6 months, -20 to -70 °C under sterile conditions after opening.
Submitted references Covalent ISG15 conjugation to CHIP promotes its ubiquitin E3 ligase activity and inhibits lung cancer cell growth in response to type I interferon.
Yoo L, Yoon AR, Yun CO, Chung KC
Cell death & disease 2018 Jan 24;9(2):97
Cell death & disease 2018 Jan 24;9(2):97
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Immunoprecipitation A549 cells transfected with HA-ISG15 were treated briefly with IFN gamma or carrier only. 500 µg of cell lysates were subjected to immunoprecipitation (IP) with 20 µg A-830 using Protein G Sepharose. IP’d material was resolved on reducing SDS-PAGE gels and Western Blots were developed using PVDF membranes and anti-HA epitope (R&D Systems MAB060) at 0.5 µg/ml followed by HRP-labeled anti-mouse (R&D Systems # HAF007) secondary antibody at 1:2000 dilution. Free (unconjugated) HA-ISG15 was visualized in both treated and untreated cells, while ISGylated proteins were clearly recovered (IP’d) from the interferon-treated cell lysates.