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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- R1195 - Provider product page
- Provider
- OriGene
- Product name
- ISG15 rabbit polyclonal antibody, Purified
- Antibody type
- Polyclonal
- Description
- ISG15 rabbit polyclonal antibody, Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- ISG15
- Antibody clone number
- NULL
- Vial size
- 500 µg
- Concentration
- 5.0 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoblot of hISG15 fusion protein. Anti-hISG15 antibody, generated by immunization with recombinant human ISG15, was tested by immunoblot against a hISG15-GFP fusion protein produced in E.coli cell lysate soluble fraction. Dilution of the antibody between 1:200 and 1:1,000 showed strong reactivity specifically with hISG15 and ISG15 coupled proteins. Free hISG15 is indicated by the arrowhead. In this blot the antibody was used at a 1:200 dilution incubated overnight at 4¡ã C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRPlabeled Donkey anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results. Data contributed by M.Malakhov, personal
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Conjugation pathways for ubiquitin and ubiquitin like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as poly-ubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1 / cullin-1 / Fbox protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15)resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch, see references above.
- Validation comment
- Assay
