Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
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Validation data
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- Product number
- PB9950 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-ISG15 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for UCRP/ISG15 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. UCRP/ISG15 information: Molecular Weight: 17888 MW; Subcellular Localization: Cytoplasm . Secreted . Exists in three distinct states: free within the cell, released into the extracellular space, or conjugated to target proteins; Tissue Specificity: Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons. Expressed in neutrophils, monocytes and lymphocytes. Enhanced expression seen in pancreatic adenocarcinoma, endometrial cancer, and bladder cancer, as compared to non-cancerous tissue. In bladder cancer, the increase in expression exhibits a striking positive correlation with more advanced stages of the disease.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of ISG15 expression in 22RV1 whole cell lysates (lane 1) and HELA whole cell lysates (lane 2). ISG15 at 15KD was detected using rabbit anti- ISG15 Antigen Affinity purified polyclonal antibody (Catalog # PB9950) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
- Additional image