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Western blot
Other assayAntibody data
- Antibody Data
- Antigen structure
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- Product number
- PA5-79523 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-ISG15 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Add 0.2 mL of distilled water, will yield a concentration of 500 µg/mL.
- Reactivity
- Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- Lot Dependent
- Storage
- -20°C
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunofluorescence analysis of ISG15 was performed using 70% confluent log phase A549 cells treated with IFN alpha (1000U/ml for 48h). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ISG15 Rabbit Polyclonal Antibody (Product # PA5-79523) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing increased ISG15 expression and localization to nucleus and cytoplasm. Panel e shows untreated cells with lower expression of ISG15. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
