Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-11268 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ISG15 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ISG15 (arrow) using an ISG15 polyclonal antibody (Product # PA5-11268) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the ISG15 gene (Lane 2).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ISG15 using ISG15 polyclonal antibody (Product # PA5-11268) in Jurkat, K562 and AML-2 cell lysates treated with chloroquine (CQ) at 0, 10, 20 and 30uM for 24 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using an ISG15 polyclonal antibody (Product # PA5-11268) in 293 cell lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cancer tissue using an ISG15 polyclonal antibody (Product # PA5-11268), followed by HRP-conjugated secondary antibody and DAB staining.