PA5-17461

antibody from Invitrogen Antibodies

Targeting: ISG15 G1P2, IFI15, UCRP

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-17461

Provider

Invitrogen Antibodies

Product name

ISG15 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody. This antibody is not cross-reactive with other ubiquitin family members, including ubiquitin, SUMO1, SUMO2, SUMO3 and NEDD8.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

34 µg/mL

Storage

-20°C

References

Submitted references

Stromal Cells Covering Omental Fat-Associated Lymphoid Clusters Trigger Formation of Neutrophil Aggregates to Capture Peritoneal Contaminants.

Jackson-Jones LH, Smith P, Portman JR, Magalhaes MS, Mylonas KJ, Vermeren MM, Nixon M, Henderson BEP, Dobie R, Vermeren S, Denby L, Henderson NC, Mole DJ, Bénézech C
Immunity 2020 Apr 14;52(4):700-715.e6

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ISG15 in lysates from HeLa cells, treated with or without IFN (1000 U/mL) for 24 hours using ISG15 polyclonal antibody (Product # PA5-17461).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane 1), A549 treated with IFNa (1000U/mL for 24 hours) (Lane 2), HeLa (Lane 3), HeLa treated with IFNa (1000U/mL for 24 hours) (Lane 4) and MCF7 (Lane 5). The blot was blocked with 2% BSA for 1h, probed with Anti-ISG15 Polyclonal Antibody (Product # PA5-17461, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 17kDa band corresponding to ISG15 was observed across all the cell lines tested and was induced upon treatment of A549 and HeLa cells with IFNa treatment.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 FALCs Are Covered by a Differentiated Monolayer of Mesothelial Cells (A) 3D reconstruction of a large portion of the omentum obtained by imaging of wholemount staining of the omentum showing omFALCs with DAPI (white). (B and C) Confocal imaging and 3D reconstruction of an omFALC showing a view of the surface of the cluster and a z section (along the dotted line) of the cluster (B) and of the surface of the omentum (C) with PDPN (red), CXCL13 (green), DAPI (blue), and IgM (yellow). (D-F) Representative confocal imaging and 3D reconstruction of an omFALC showing a view of the surface of the cluster and a z section (dotted line) of the cluster with PDPN (red); ENPP2 (D, green), Cathepsin-C (E, CTSC, green), or ISG15 (F, green); DAPI (blue) and IgM (D, yellow); or CD45 (E and F, yellow). The surface of the cluster and the mesothelium are delimited by a hyphenated line. (G) Violin plots of gene expression of inflammatory chemokines by cluster with highest log-normalized expression value labeled. (H) Amounts of CCL2, CXCL10, and CXCL1 secreted into the supernatant of 2-h omentum explant culture per omentum and per mL after exposure to Zymosan-A for 2 h either in vivo (i.p. injection) or ex vivo . Data pooled from two independent experiments with n = 10 mice per group. (I) Confocal imaging and 3D reconstruction of omFALC showing the surface of omFALCs (first column) and a clipped view inside the cluster (second column). Scale bars, 100 mum. All staining representative of n