Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP11162PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP11162PU-N, RRID:AB_1769230
- Product name
- anti ISG15 / G1P2 (N-term)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 39~68 amino acids from the N-terminal region of Human ISG15.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of anti-ISG15 Pab (Cat.#AP11162PU-N) to detect ISG15 in mouse kidney tissue lysate.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of ISG15 (arrow) using Rabbit polyclonal hISG15-A46 (Cat. #AP11162PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ISG15 gene (Lane 2)
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis in 293 cell line lysates (35ug/lane) using ISG15 Antibody (A46) (Cat.#AP11162PU-N). This demonstrates the ISG15 antibody detected the ISG15 protein (arrow).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis using ISG15 Antibody in Jurkat? K562 and AML-2 cell line lysates treated with a small molecular of 0?10?20?30uM for 24 hours. Offered friendly by Biyin Cao, Soochow University, Center for Blood Research.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.