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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [9]
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- Validations
- Western blot [1]
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- Product number
- HPA004627 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA004627, RRID:AB_1079152
- Product name
- Anti-ISG15
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human ISG15, Gene description: ISG15 ubiquitin-like modifier, Alternative Gene Names: G1P2, IFI15, UCRP, Validated applications: WB, Uniprot ID: P05161, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references SIRT1 ISGylation accelerates tumor progression by unleashing SIRT1 from the inactive state to promote its deacetylase activity.
Dynamic molecular network analysis of iPSC-Purkinje cells differentiation delineates roles of ISG15 in SCA1 at the earliest stage.
Famotidine inhibits toll-like receptor 3-mediated inflammatory signaling in SARS-CoV-2 infection
Expression of Ki-67, Cornulin and ISG15 in non-involved mucosal surgical margins as predictive markers for relapse in oral squamous cell carcinoma (OSCC)
Papain-like protease regulates SARS-CoV-2 viral spread and innate immunity
Treatment with Commonly Used Antiretroviral Drugs Induces a Type I/III Interferon Signature in the Gut in the Absence of HIV Infection.
TLR7 agonist administration to SIV-infected macaques receiving early initiated cART does not induce plasma viremia
Immune clearance of highly pathogenic SIV infection
Downregulation of Robust Acute Type I Interferon Responses Distinguishes Nonpathogenic Simian Immunodeficiency Virus (SIV) Infection of Natural Hosts from Pathogenic SIV Infection of Rhesus Macaques
Kang JA, Kim YJ, Jang KY, Moon HW, Lee H, Lee S, Song HK, Cho SW, Yoo YS, Han HG, Kim MJ, Chung MJ, Choi CY, Lee C, Chung C, Hur GM, Kim YS, Jeon YJ
Experimental & molecular medicine 2024 Mar;56(3):656-673
Experimental & molecular medicine 2024 Mar;56(3):656-673
Dynamic molecular network analysis of iPSC-Purkinje cells differentiation delineates roles of ISG15 in SCA1 at the earliest stage.
Homma H, Yoshioka Y, Fujita K, Shirai S, Hama Y, Komano H, Saito Y, Yabe I, Okano H, Sasaki H, Tanaka H, Okazawa H
Communications biology 2024 Apr 9;7(1):413
Communications biology 2024 Apr 9;7(1):413
Famotidine inhibits toll-like receptor 3-mediated inflammatory signaling in SARS-CoV-2 infection
Mukherjee R, Bhattacharya A, Bojkova D, Mehdipour A, Shin D, Khan K, Hei-Yin Cheung H, Wong K, Ng W, Cinatl J, Geurink P, van der Heden van Noort G, Rajalingam K, Ciesek S, Hummer G, Dikic I
Journal of Biological Chemistry 2021;297(2):100925
Journal of Biological Chemistry 2021;297(2):100925
Expression of Ki-67, Cornulin and ISG15 in non-involved mucosal surgical margins as predictive markers for relapse in oral squamous cell carcinoma (OSCC)
Ha P, Govindaraj P, Kallarakkal T, Mohd Zain R, Tilakaratne W, Lew H
PLOS ONE 2021;16(12):e0261575
PLOS ONE 2021;16(12):e0261575
Papain-like protease regulates SARS-CoV-2 viral spread and innate immunity
Shin D, Mukherjee R, Grewe D, Bojkova D, Baek K, Bhattacharya A, Schulz L, Widera M, Mehdipour A, Tascher G, Geurink P, Wilhelm A, van der Heden van Noort G, Ovaa H, Müller S, Knobeloch K, Rajalingam K, Schulman B, Cinatl J, Hummer G, Ciesek S, Dikic I
Nature 2020;587(7835):657-662
Nature 2020;587(7835):657-662
Treatment with Commonly Used Antiretroviral Drugs Induces a Type I/III Interferon Signature in the Gut in the Absence of HIV Infection.
Hughes SM, Levy CN, Calienes FL, Stekler JD, Pandey U, Vojtech L, Berard AR, Birse K, Noël-Romas L, Richardson B, Golden JB, Cartwright M, Collier AC, Stevens CE, Curlin ME, Holtz TH, Mugo N, Irungu E, Katabira E, Muwonge T, Lama JR, Baeten JM, Burgener A, Lingappa JR, McElrath MJ, Mackelprang R, McGowan I, Cranston RD, Cameron MJ, Hladik F
Cell reports. Medicine 2020 Sep 22;1(6):100096
Cell reports. Medicine 2020 Sep 22;1(6):100096
TLR7 agonist administration to SIV-infected macaques receiving early initiated cART does not induce plasma viremia
Del Prete G, Alvord W, Li Y, Deleage C, Nag M, Oswald K, Thomas J, Pyle C, Bosche W, Coalter V, Wiles A, Wiles R, Berkemeier B, Hull M, Chipriano E, Silipino L, Fast R, Kiser J, Kiser R, Malys T, Kramer J, Breed M, Trubey C, Estes J, Barnes T, Hesselgesser J, Geleziunas R, Lifson J
JCI Insight 2019;4(11)
JCI Insight 2019;4(11)
Immune clearance of highly pathogenic SIV infection
Hansen S, Jr M, Ventura A, Hughes C, Gilbride R, Ford J, Oswald K, Shoemaker R, Li Y, Lewis M, Gilliam A, Xu G, Whizin N, Burwitz B, Planer S, Turner J, Legasse A, Axthelm M, Nelson J, Früh K, Sacha J, Estes J, Keele B, Edlefsen P, Lifson J, Picker L
Nature 2013;502(7469):100-104
Nature 2013;502(7469):100-104
Downregulation of Robust Acute Type I Interferon Responses Distinguishes Nonpathogenic Simian Immunodeficiency Virus (SIV) Infection of Natural Hosts from Pathogenic SIV Infection of Rhesus Macaques
Harris L, Tabb B, Sodora D, Paiardini M, Klatt N, Douek D, Silvestri G, Müller-Trutwin M, Vasile-Pandrea I, Apetrei C, Hirsch V, Lifson J, Brenchley J, Estes J
Journal of Virology 2010;84(15):7886-7891
Journal of Virology 2010;84(15):7886-7891
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and ISG15 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY417516).
- Sample type
- Human
- Protocol
- Protocol
