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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunohistochemistry [3]
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- Product number
- NB100-79823 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-79823, RRID:AB_1107988
- Product name
- Rabbit Polyclonal CPSF2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references THOC5 controls 3'end-processing of immediate early genes via interaction with polyadenylation specific factor 100 (CPSF100).
Nucleophosmin deposition during mRNA 3' end processing influences poly(A) tail length.
Tran DD, Saran S, Williamson AJ, Pierce A, Dittrich-Breiholz O, Wiehlmann L, Koch A, Whetton AD, Tamura T
Nucleic acids research 2014 Oct 29;42(19):12249-60
Nucleic acids research 2014 Oct 29;42(19):12249-60
Nucleophosmin deposition during mRNA 3' end processing influences poly(A) tail length.
Sagawa F, Ibrahim H, Morrison AL, Wilusz CJ, Wilusz J
The EMBO journal 2011 Aug 5;30(19):3994-4005
The EMBO journal 2011 Aug 5;30(19):3994-4005
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CPSF2 Antibody [NB100-79823] - Detection of CPSF2 from Hela whole cell lysate (5, 15, 50 microgram), 293T (T; 50 microgram) and mouse NIH3T3 (M; 50 microgram). CPSF100 was also immunoprecipitated by rabbit anti-CPSF100 antibodies NB100-79824 and NB100-79825.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CPSF2 Antibody [NB100-79823] - Human pancreatic islet cell tumor (left) and mouse teratoma (right). Antibody used at a dilution of 1:200 (1ug/ml).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: CPSF2 Antibody [NB100-79823] - FFPE section of human ovarian carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: CPSF2 Antibody [NB100-79823] - FFPE section of mouse teratoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.
