16004-1-AP

antibody from Proteintech Group

Targeting: NBR1 1A1-3B, CA125, KIAA0049, M17S2

Provider product page for 16004-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [47]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunoprecipitation [1]
Immunohistochemistry [2]

Submit

Product number: 16004-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#16004-1-AP, RRID:AB_2251178
Product name: NBR1 antibody
Antibody type: Polyclonal
Description: KD/KO validated NBR1 antibody (Cat. #16004-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat, monkey and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat, Simian
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

NBR1 protein structure - 16004-1-AP shown in red.

Submitted references Maternal prednisone exposure during pregnancy elevates susceptibility to osteoporosis in female offspring: The role of mitophagy/FNDC5 alteration in skeletal muscle.

Du ZY, Zhu HL, Chang W, Zhang YF, Ling Q, Wang KW, Zhang J, Zhang QB, Kan XL, Wang QN, Wang H, Zhou Y
Journal of hazardous materials 2024 May 5;469:133997

NBR1-dependent autophagy activation protects against environmental cadmium-evoked placental trophoblast senescence.

Ling Q, Zhang YF, Chang W, Liu ST, Zhu HL, Wang H
Chemosphere 2024 Jun;358:142138

TFEB activation triggers pexophagy for functional adaptation during oxidative stress under calcium deficient-conditions.

Manandhar L, Dutta RK, Devkota P, Chhetri A, Wei X, Park C, Kwon HM, Park R
Cell communication and signaling : CCS 2024 Feb 21;22(1):142

Enteric coronavirus nsp2 is a virulence determinant that recruits NBR1 for autophagic targeting of TBK1 to diminish the innate immune response.

Jiao Y, Zhao P, Xu LD, Yu JQ, Cai HL, Zhang C, Tong C, Yang YL, Xu P, Sun Q, Chen N, Wang B, Huang YW
Autophagy 2024 Aug;20(8):1762-1779

NBR1-p62-Nrf2 mediates the anti-pulmonary fibrosis effects of protodioscin.

Zeng Q, Wen BB, Liu X, Luo YY, Hu ZG, Huang L, Zhang XH, Huang XT, Zhou TT, Sang XX, Luo YY, Xiong DY, Luo ZQ, Liu W, Tang SY
Chinese medicine 2024 Apr 8;19(1):60

TOLLIP-mediated autophagic degradation pathway links the VCP-TMEM63A-DERL1 signaling axis to triple-negative breast cancer progression.

Zhang TM, Liao L, Yang SY, Huang MY, Zhang YL, Deng L, Hu SY, Yang F, Zhang FL, Shao ZM, Li DQ
Autophagy 2023 Mar;19(3):805-821

Stress granule homeostasis is modulated by TRIM21-mediated ubiquitination of G3BP1 and autophagy-dependent elimination of stress granules.

Yang C, Wang Z, Kang Y, Yi Q, Wang T, Bai Y, Liu Y
Autophagy 2023 Jul;19(7):1934-1951

PSMB1 Inhibits the Replication of Porcine Reproductive and Respiratory Syndrome Virus by Recruiting NBR1 To Degrade Nonstructural Protein 12 by Autophagy.

Li L, Bai Y, Zhou Y, Jiang Y, Tong W, Li G, Zheng H, Gao F, Tong G
Journal of virology 2023 Jan 31;97(1):e0166022

Chloroquine is a safe autophagy inhibitor for sustaining the expression of antioxidant enzymes in trophoblasts.

Furuta A, Shima T, Yoshida-Kawaguchi M, Yamada K, Yasuda I, Tsuda S, Yamaki-Ushijima A, Yoneda S, Higashisaka K, Cheng SB, Matsumoto K, Tsutsumi Y, Sharma S, Saito S, Nakashima A
Journal of reproductive immunology 2023 Feb;155:103766

Enhanced liquidity of p62 droplets mediated by Smurf1 links Nrf2 activation and autophagy.

Xia Q, Li Y, Xu W, Wu C, Zheng H, Liu L, Dong L
Cell & bioscience 2023 Feb 21;13(1):37

The nonstructural protein 1 of respiratory syncytial virus hijacks host mitophagy as a novel mitophagy receptor to evade the type I IFN response in HEp-2 cells.

Cheng J, Wang Y, Yin L, Liang W, Zhang J, Ma C, Zhang Y, Liu B, Wang J, Zhao W, Li M, Wei L
mBio 2023 Dec 19;14(6):e0148023

The Troyer syndrome protein spartin mediates selective autophagy of lipid droplets.

Chung J, Park J, Lai ZW, Lambert TJ, Richards RC, Zhang J, Walther TC, Farese RV Jr
Nature cell biology 2023 Aug;25(8):1101-1110

Stratification of diabetic kidney diseases via data-independent acquisition proteomics-based analysis of human kidney tissue specimens.

Huang Q, Fei X, Zhong Z, Zhou J, Gong J, Chen Y, Li Y, Wu X
Frontiers in endocrinology 2022;13:995362

NBR1 mediates autophagic degradation of IRF3 to negatively regulate type I interferon production.

Cai Y, Zhu Y, Zheng J, Zhang Y, Chen W
Biochemical and biophysical research communications 2022 Oct 1;623:140-147

Increased CSF-decorin predicts brain pathological changes driven by Alzheimer's Aβ amyloidosis.

Jiang R, Smailovic U, Haytural H, Tijms BM, Li H, Haret RM, Shevchenko G, Chen G, Abelein A, Gobom J, Frykman S, Sekiguchi M, Fujioka R, Watamura N, Sasaguri H, Nyström S, Hammarström P, Saido TC, Jelic V, Syvänen S, Zetterberg H, Winblad B, Bergquist J, Visser PJ, Nilsson P
Acta neuropathologica communications 2022 Jul 4;10(1):96

A detrimental role of NLRP6 in host iron metabolism during Salmonella infection.

Deng Q, Yang S, Sun L, Huang K, Dong K, Zhu Y, Cao Y, Li Y, Wu S, Huang R
Redox biology 2022 Feb;49:102217

Roux-en-Y Gastric Bypass Modulates AMPK, Autophagy and Inflammatory Response in Leukocytes of Obese Patients.

Abad-Jiménez Z, López-Domènech S, García-Gargallo C, Vezza T, Gómez-Abril SÁ, Morillas C, Díaz-Pozo P, Falcón R, Bañuls C, Víctor VM, Rocha M
Biomedicines 2022 Feb 12;10(2)

Elevated Expression of MiR-17 in Microglia of Alzheimer's Disease Patients Abrogates Autophagy-Mediated Amyloid-β Degradation.

Estfanous S, Daily KP, Eltobgy M, Deems NP, Anne MNK, Krause K, Badr A, Hamilton K, Carafice C, Hegazi A, Abu Khweek A, Kelani H, Nimjee S, Awad H, Zhang X, Cormet-Boyaka E, Haffez H, Soror S, Mikhail A, Nuovo G, Barrientos RM, Gavrilin MA, Amer AO
Frontiers in immunology 2021;12:705581

Asparaginyl endopeptidase (AEP) regulates myocardial apoptosis in response to radiation exposure via alterations in NRF2 activation.

Cao L, Xu C, Yi P, Li H, Lin Y, Cai G, Wang S, Ou D, Li M, Chen J
American journal of cancer research 2021;11(4):1206-1225

Autophagy receptor OPTN (optineurin) regulates mesenchymal stem cell fate and bone-fat balance during aging by clearing FABP3.

Liu ZZ, Hong CG, Hu WB, Chen ML, Duan R, Li HM, Yue T, Cao J, Wang ZX, Chen CY, Hu XK, Wu B, Liu HM, Tan YJ, Liu JH, Luo ZW, Zhang Y, Rao SS, Luo MJ, Yin H, Wang YY, Xia K, Xu L, Tang SY, Hu RG, Xie H
Autophagy 2021 Oct;17(10):2766-2782

Macroautophagy supports Sonic Hedgehog signaling by promoting Patched1 degradation.

Yang X, Jin N, Wang Y, Yao Y, Wang Y, Li T, Liu C, Yu T, Yin H, Zhang Z, Cheng SY, Yue S
Biochimica et biophysica acta. Molecular cell research 2021 Nov;1868(12):119124

Galectin-9 Targets NLRP3 for Autophagic Degradation to Limit Inflammation.

Wang W, Qin Y, Song H, Wang L, Jia M, Zhao C, Gong M, Zhao W
Journal of immunology (Baltimore, Md. : 1950) 2021 Jun 1;206(11):2692-2699

Knockdown of PEX16 Induces Autophagic Degradation of Peroxisomes.

Wei X, Maharjan Y, Dorotea D, Dutta RK, Kim D, Kim H, Mu Y, Park C, Park R
International journal of molecular sciences 2021 Jul 26;22(15)

METTL14 facilitates global genome repair and suppresses skin tumorigenesis.

Yang Z, Yang S, Cui YH, Wei J, Shah P, Park G, Cui X, He C, He YY
Proceedings of the National Academy of Sciences of the United States of America 2021 Aug 31;118(35)

ALS-FTLD-linked mutations of SQSTM1/p62 disrupt selective autophagy and NFE2L2/NRF2 anti-oxidative stress pathway.

Deng Z, Lim J, Wang Q, Purtell K, Wu S, Palomo GM, Tan H, Manfredi G, Zhao Y, Peng J, Hu B, Chen S, Yue Z
Autophagy 2020 May;16(5):917-931

Microglia clear neuron-released α-synuclein via selective autophagy and prevent neurodegeneration.

Choi I, Zhang Y, Seegobin SP, Pruvost M, Wang Q, Purtell K, Zhang B, Yue Z
Nature communications 2020 Mar 13;11(1):1386

Dimethyloxaloylglycine induces pexophagy in a HIF-2α dependent manner involving autophagy receptor p62.

Mu Y, Maharjan Y, Dutta RK, Kim H, Wei X, Kim JH, Kim D, Park C, Park R
Biochemical and biophysical research communications 2020 Feb 16;

An HMGA2-p62-ERα axis regulates uterine leiomyomas proliferation.

Liu B, Chen G, He Q, Liu M, Gao K, Cai B, Qu J, Lin S, Geng A, Li S, Wang K, Mao Z, Wan X, Yan Q
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Aug;34(8):10966-10983

GABARAPL2 Is Critical for Growth Restriction of Toxoplasma gondii in HeLa Cells Treated with Gamma Interferon.

Zhang Z, Gu H, Li Q, Zheng J, Cao S, Weng C, Jia H
Infection and immunity 2020 Apr 20;88(5)

The pharmalogical reactivation of p53 function improves breast tumor cell lysis by granzyme B and NK cells through induction of autophagy.

Chollat-Namy M, Ben Safta-Saadoun T, Haferssas D, Meurice G, Chouaib S, Thiery J
Cell death & disease 2019 Sep 20;10(10):695

Mitochondrial dysfunction and mitophagy defect triggered by heterozygous GBA mutations.

Li H, Ham A, Ma TC, Kuo SH, Kanter E, Kim D, Ko HS, Quan Y, Sardi SP, Li A, Arancio O, Kang UJ, Sulzer D, Tang G
Autophagy 2019 Jan;15(1):113-130

Electrophysiological and histopathological findings of muscular disease suspected as myotonic dystrophy in a Shiba dog.

Shiga T, Okuno S, Uchida K, Chambers JK, Nakayama H
The Journal of veterinary medical science 2018 Mar 24;80(3):480-484

Catalase inhibition induces pexophagy through ROS accumulation.

Lee JN, Dutta RK, Maharjan Y, Liu ZQ, Lim JY, Kim SJ, Cho DH, So HS, Choe SK, Park R
Biochemical and biophysical research communications 2018 Jun 27;501(3):696-702

Autophagy defects and related genetic variations in renal cell carcinoma with eosinophilic cytoplasmic inclusions.

Yu Z, Ma J, Li X, Liu Y, Li M, Wang L, Zhao M, He H, Zhang Y, Rao Q, Zhao D, Wang Y, Fan L, Li P, Liu Y, Liu F, Zhang F, Ye J, Yan Q, Guo S, Wang Z
Scientific reports 2018 Jul 2;8(1):9972

Modulation of Protein Quality Control and Proteasome to Autophagy Switch in Immortalized Myoblasts from Duchenne Muscular Dystrophy Patients.

Wattin M, Gaweda L, Muller P, Baritaud M, Scholtes C, Lozano C, Gieseler K, Kretz-Remy C
International journal of molecular sciences 2018 Jan 7;19(1)

The HECT E3 ubiquitin ligase NEDD4 interacts with and ubiquitylates SQSTM1 for inclusion body autophagy.

Lin Q, Dai Q, Meng H, Sun A, Wei J, Peng K, Childress C, Chen M, Shao G, Yang W
Journal of cell science 2017 Nov 15;130(22):3839-3850

Export-deficient monoubiquitinated PEX5 triggers peroxisome removal in SV40 large T antigen-transformed mouse embryonic fibroblasts.

Nordgren M, Francisco T, Lismont C, Hennebel L, Brees C, Wang B, Van Veldhoven PP, Azevedo JE, Fransen M
Autophagy 2015;11(8):1326-40

Lack of Neuronal IFN-β-IFNAR Causes Lewy Body- and Parkinson's Disease-like Dementia.

Ejlerskov P, Hultberg JG, Wang J, Carlsson R, Ambjørn M, Kuss M, Liu Y, Porcu G, Kolkova K, Friis Rundsten C, Ruscher K, Pakkenberg B, Goldmann T, Loreth D, Prinz M, Rubinsztein DC, Issazadeh-Navikas S
Cell 2015 Oct 8;163(2):324-39

The origin and role of autophagy in the formation of cytoplasmic granules in canine lingual granular cell tumors.

Suzuki S, Uchida K, Harada T, Nibe K, Yamashita M, Ono K, Nakayama H
Veterinary pathology 2015 May;52(3):456-64

Identification of a lung cancer cell line deficient in atg7-dependent autophagy.

Mandelbaum J, Rollins N, Shah P, Bowman D, Lee JY, Tayber O, Bernard H, LeRoy P, Li P, Koenig E, Brownell JE, D'Amore N
Autophagy 2015 Jun 19;:0

Hif-2α promotes degradation of mammalian peroxisomes by selective autophagy.

Walter KM, Schönenberger MJ, Trötzmüller M, Horn M, Elsässer HP, Moser AB, Lucas MS, Schwarz T, Gerber PA, Faust PL, Moch H, Köfeler HC, Krek W, Kovacs WJ
Cell metabolism 2014 Nov 4;20(5):882-897

LC3, an autophagosome marker, is expressed on oligodendrocytes in Nasu-Hakola disease brains.

Satoh J, Motohashi N, Kino Y, Ishida T, Yagishita S, Jinnai K, Arai N, Nakamagoe K, Tamaoka A, Saito Y, Arima K
Orphanet journal of rare diseases 2014 May 1;9:68

Ubiquitin-negative, eosinophilic neuronal cytoplasmic inclusions associated with stress granules and autophagy: an immunohistochemical investigation of two cases.

Mori F, Watanabe Y, Miki Y, Tanji K, Odagiri S, Eto K, Wakabayashi K
Neuropathology : official journal of the Japanese Society of Neuropathology 2014 Apr;34(2):140-7

Giant cell polymyositis and myocarditis associated with myasthenia gravis and thymoma.

Kon T, Mori F, Tanji K, Miki Y, Kimura T, Wakabayashi K
Neuropathology : official journal of the Japanese Society of Neuropathology 2013 Jun;33(3):281-7

p62/sequestosome 1 binds to TDP-43 in brains with frontotemporal lobar degeneration with TDP-43 inclusions.

Tanji K, Zhang HX, Mori F, Kakita A, Takahashi H, Wakabayashi K
Journal of neuroscience research 2012 Oct;90(10):2034-42

Autophagic adapter protein NBR1 is localized in Lewy bodies and glial cytoplasmic inclusions and is involved in aggregate formation in α-synucleinopathy.

Odagiri S, Tanji K, Mori F, Kakita A, Takahashi H, Wakabayashi K
Acta neuropathologica 2012 Aug;124(2):173-86

Mori F, Tanji K, Odagiri S, Toyoshima Y, Yoshida M, Kakita A, Takahashi H, Wakabayashi K
Neuroscience letters 2012 Aug 1;522(2):134-8

No comments: Submit comment

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 16004-1-AP(NBR1 antibody) at dilution of 1:1500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using NBR1 antibody 16004-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP Result of anti-NBR1 (IP:16004-1-AP, 4ug; Detection:16004-1-AP 1:800) with HeLa cells lysate 2500ug.
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human breast cancer using 16004-1-AP(NBR1 antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human breast cancer using 16004-1-AP(NBR1 antibody) at dilution of 1:50 (under 40x lens)
Sample type: tissue