16004-1-AP

antibody from Proteintech Group

Targeting: NBR1 1A1-3B, CA125, KIAA0049, M17S2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

16004-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#16004-1-AP, RRID:AB_2251178

Product name

NBR1 antibody

Antibody type

Polyclonal

Description

KD/KO validated NBR1 antibody (Cat. #16004-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat, monkey and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat, Simian

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

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Autophagy receptor OPTN (optineurin) regulates mesenchymal stem cell fate and bone-fat balance during aging by clearing FABP3.

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Macroautophagy supports Sonic Hedgehog signaling by promoting Patched1 degradation.

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Galectin-9 Targets NLRP3 for Autophagic Degradation to Limit Inflammation.

Wang W, Qin Y, Song H, Wang L, Jia M, Zhao C, Gong M, Zhao W
Journal of immunology (Baltimore, Md. : 1950) 2021 Jun 1;206(11):2692-2699

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Knockdown of PEX16 Induces Autophagic Degradation of Peroxisomes.

Wei X, Maharjan Y, Dorotea D, Dutta RK, Kim D, Kim H, Mu Y, Park C, Park R
International journal of molecular sciences 2021 Jul 26;22(15)

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METTL14 facilitates global genome repair and suppresses skin tumorigenesis.

Yang Z, Yang S, Cui YH, Wei J, Shah P, Park G, Cui X, He C, He YY
Proceedings of the National Academy of Sciences of the United States of America 2021 Aug 31;118(35)

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ALS-FTLD-linked mutations of SQSTM1/p62 disrupt selective autophagy and NFE2L2/NRF2 anti-oxidative stress pathway.

Deng Z, Lim J, Wang Q, Purtell K, Wu S, Palomo GM, Tan H, Manfredi G, Zhao Y, Peng J, Hu B, Chen S, Yue Z
Autophagy 2020 May;16(5):917-931

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Microglia clear neuron-released α-synuclein via selective autophagy and prevent neurodegeneration.

Choi I, Zhang Y, Seegobin SP, Pruvost M, Wang Q, Purtell K, Zhang B, Yue Z
Nature communications 2020 Mar 13;11(1):1386

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Dimethyloxaloylglycine induces pexophagy in a HIF-2α dependent manner involving autophagy receptor p62.

Mu Y, Maharjan Y, Dutta RK, Kim H, Wei X, Kim JH, Kim D, Park C, Park R
Biochemical and biophysical research communications 2020 Feb 16;

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An HMGA2-p62-ERα axis regulates uterine leiomyomas proliferation.

Liu B, Chen G, He Q, Liu M, Gao K, Cai B, Qu J, Lin S, Geng A, Li S, Wang K, Mao Z, Wan X, Yan Q
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GABARAPL2 Is Critical for Growth Restriction of Toxoplasma gondii in HeLa Cells Treated with Gamma Interferon.

Zhang Z, Gu H, Li Q, Zheng J, Cao S, Weng C, Jia H
Infection and immunity 2020 Apr 20;88(5)

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The pharmalogical reactivation of p53 function improves breast tumor cell lysis by granzyme B and NK cells through induction of autophagy.

Chollat-Namy M, Ben Safta-Saadoun T, Haferssas D, Meurice G, Chouaib S, Thiery J
Cell death & disease 2019 Sep 20;10(10):695

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Mitochondrial dysfunction and mitophagy defect triggered by heterozygous GBA mutations.

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Autophagy 2019 Jan;15(1):113-130

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Electrophysiological and histopathological findings of muscular disease suspected as myotonic dystrophy in a Shiba dog.

Shiga T, Okuno S, Uchida K, Chambers JK, Nakayama H
The Journal of veterinary medical science 2018 Mar 24;80(3):480-484

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Catalase inhibition induces pexophagy through ROS accumulation.

Lee JN, Dutta RK, Maharjan Y, Liu ZQ, Lim JY, Kim SJ, Cho DH, So HS, Choe SK, Park R
Biochemical and biophysical research communications 2018 Jun 27;501(3):696-702

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Autophagy defects and related genetic variations in renal cell carcinoma with eosinophilic cytoplasmic inclusions.

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Modulation of Protein Quality Control and Proteasome to Autophagy Switch in Immortalized Myoblasts from Duchenne Muscular Dystrophy Patients.

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Journal of cell science 2017 Nov 15;130(22):3839-3850

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Autophagy 2015;11(8):1326-40

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Lack of Neuronal IFN-β-IFNAR Causes Lewy Body- and Parkinson's Disease-like Dementia.

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Autophagic adapter protein NBR1 is localized in Lewy bodies and glial cytoplasmic inclusions and is involved in aggregate formation in α-synucleinopathy.

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Autophagy-related proteins (p62, NBR1 and LC3) in intranuclear inclusions in neurodegenerative diseases.

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Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 16004-1-AP(NBR1 antibody) at dilution of 1:1500

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using NBR1 antibody 16004-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-NBR1 (IP:16004-1-AP, 4ug; Detection:16004-1-AP 1:800) with HeLa cells lysate 2500ug.

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human breast cancer using 16004-1-AP(NBR1 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human breast cancer using 16004-1-AP(NBR1 antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue