Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AF6269 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal DCBLD2/ESDN Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human and mouse DCBLD2/ESDN in Western blots and human DCBLD2/ESDN in direct ELISAs.
- Reactivity
- Human, Mouse
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse DCBLD2/ESDN by Western Blot. Western blot shows lysates of P19 mouse embryonal carcinoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Human/Mouse DCBLD2/ESDN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6269) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for DCBLD2/ESDN at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of DCBLD2/ESDN in NCI-H460 Human Cell Line by Flow Cytometry. NCI-H460 human large cell lung carcinoma cell line was stained with Human/Mouse DCBLD2/ESDN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6269, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).