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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- ARP38332_P050 - Provider product page
- Provider
- Aviva Systems Biology
- Proper citation
- Aviva Systems Biology Cat#ARP38332_P050, RRID:AB_10568858
- Product name
- ZNF133 antibody - middle region (ARP38332_P050)
- Antibody type
- Polyclonal
- Antigen
- The immunogen is a synthetic peptide directed towards the middle region of human ZNF133
- Description
- Affinity Purified
- Reactivity
- Human, Porcine, Rabbit
- Host
- Rabbit
- Vial size
- 100 μl
- Concentration
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- Storage
- For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- WB Suggested Anti-ZNF133Antibody Titration: 0.2-1µg/ml. ELISA Titer: 1:312500Positive Control: THP-1 cell lysate
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Three cryosections on each slide were stained with a single antibody. The fourth slice was used as a negative control (primary antibody omitted). The tissue was fixed with cold acetone for 10 minutes, rinsed in phosphate buffered saline (PBS) and incubated with the primary antibody diluted in 1% bovine serum albumin (BSA) in PBS for 1 hour at room temperature.Rabbit anti human antibodies and dilutions used:e) ZNF133 (38332), most suitable concentration 1:100
