Explore
Validate
Learn500-P253-1MG
antibody from Invitrogen Antibodies
Targeting: DEFB1
BD1, DEFB-1, DEFB101, HBD-1, HBD1, MGC51822
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- ELISA [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 500-P253-1MG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- beta-Defensin 1 Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: GNFLTGLGHR SDHYNCVSSG GQCLYSACPI FTKIQGTCYR GKAKCCK. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human BD-1. Anti-Human BD-1-specific antibody was purified by affinity chromatography employing an immobilized Human BD-1 matrix. Sandwich ELISA: To detect Human BD-1 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human BD-1 (500-P253Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human BD-1. Western Blot: To detect Human BD-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human BD-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 1 mg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
Submitted references β-Defensin 1 Is Prominent in the Liver and Induced During Cholestasis by Bilirubin and Bile Acids via Farnesoid X Receptor and Constitutive Androstane Receptor.
Bitter and sweet taste receptors regulate human upper respiratory innate immunity.
Klag T, Thomas M, Ehmann D, Courth L, Mailänder-Sanchez D, Weiss TS, Dayoub R, Abshagen K, Vollmar B, Thasler WE, Stange EF, Berg CP, Malek NP, Zanger UM, Wehkamp J
Frontiers in immunology 2018;9:1735
Frontiers in immunology 2018;9:1735
Bitter and sweet taste receptors regulate human upper respiratory innate immunity.
Lee RJ, Kofonow JM, Rosen PL, Siebert AP, Chen B, Doghramji L, Xiong G, Adappa ND, Palmer JN, Kennedy DW, Kreindler JL, Margolskee RF, Cohen NA
The Journal of clinical investigation 2014 Mar;124(3):1393-405
The Journal of clinical investigation 2014 Mar;124(3):1393-405
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect Human BD-1 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of beta-Defensin 1 Polyclonal Antibody (Product # 500-P253-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech beta-Defensin 1 Polyclonal Antibody, Biotin (Product # 500-P253BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human BD-1.
