ABIN951877

antibody from antibodies-online

Targeting: DEFB1 BD1, DEFB-1, DEFB101, HBD-1, HBD1, MGC51822

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN951877

Provider

antibodies-online

Product name

anti-Defensin, beta 1 (DEFB1) (N-Term), (AA 11-37) antibody

Antibody type

Polyclonal

Antigen

KLH conjugated synthetic peptide between 11-37 amino acids from the N-terminal region of Human DEFB1. Genename: DEFB1

Description

Protein A column, followed by peptide affinity purification

Reactivity

Human

Host

Rabbit

Epitope

N-Term,AA 11-37

Vial size

0.4 mL

Concentration

0.25 mg/mL

Storage

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.

Handling

Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Western blot analysis of Defensin beta 1 Antibody (N-term) Cat.-No AP51231PU-N in MDA-MB231 cell line lysates (35ug/lane). This demonstrates the DEFB1 antibody detected the DEFB1 protein (arrow).

Immunocytochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of Defensin beta 1 Antibody (N-term) Cat.-No AP51231PU-N with MDA-MB231 cell followed by Alexa Fluor^®488-conjugated Goat anti-Rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Immunohistochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Formalin fixed, paraffin embedded human kidney tissue stained with Defensin beta 1 Antibody (N-term) Cat.-No AP51231PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Flow cytometric analysis of MDA-MB231 cells using Defensin beta 1 Antibody (N-term) Cat.-No AP51231PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.