Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [4]
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Validation data
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- Product number
- NBP1-84281 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-84281, RRID:AB_11005596
- Product name
- Rabbit Polyclonal DEFA6 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human DEFA6 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references REG gene expression in inflamed and healthy colon mucosa explored by in situ hybridisation.
Macaque paneth cells express lymphoid chemokine CXCL13 and other antimicrobial peptides not previously described as expressed in intestinal crypts.
Activation of REG family proteins in colitis.
van Beelen Granlund A, Østvik AE, Brenna Ø, Torp SH, Gustafsson BI, Sandvik AK
Cell and tissue research 2013 Jun;352(3):639-46
Cell and tissue research 2013 Jun;352(3):639-46
Macaque paneth cells express lymphoid chemokine CXCL13 and other antimicrobial peptides not previously described as expressed in intestinal crypts.
Lucero CM, Fallert Junecko B, Klamar CR, Sciullo LA, Berendam SJ, Cillo AR, Qin S, Sui Y, Sanghavi S, Murphey-Corb MA, Reinhart TA
Clinical and vaccine immunology : CVI 2013 Aug;20(8):1320-8
Clinical and vaccine immunology : CVI 2013 Aug;20(8):1320-8
Activation of REG family proteins in colitis.
Granlund Av, Beisvag V, Torp SH, Flatberg A, Kleveland PM, Ostvik AE, Waldum HL, Sandvik AK
Scandinavian journal of gastroenterology 2011 Nov;46(11):1316-23
Scandinavian journal of gastroenterology 2011 Nov;46(11):1316-23
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DEFA6 Antibody [NBP1-84281] - Analysis in human small intestine tissue.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: DEFA6 Antibody [NBP1-84281] - Western lane view shows lysates of Ileum stem cells day 9 differentiated and day 12 differentiated, loaded at 0.2 mg/mL. A specific band was detected for DEFA6 at approximately 15 kDa (as indicated) using 4 ug/mL of Rabbit Anti-DEFA6 Polyclonal Antibody (Catalog # NBP1-84281). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DEFA6 Antibody [NBP1-84281] - Analysis in control (vector only transfected HEK293T lysate) and DEFA6 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DEFA6 Antibody [NBP1-84281] - Staining of human duodenum shows cytoplasmic positivity in Paneth cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DEFA6 Antibody [NBP1-84281] - Staining of human liver shows no cytoplasmic positivity in hepatocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DEFA6 Antibody [NBP1-84281] - Staining of human small intestine shows cytoplasmic positivity in Paneth cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DEFA6 Antibody [NBP1-84281] - Staining in human small intestine and liver tissues . Corresponding DEFA6 RNA-seq data are presented for the same tissues.