PA5-117076

antibody from Invitrogen Antibodies

Targeting: DEFA1 DEF1, DEFA2, HNP-1, MRS

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-117076

Provider

Invitrogen Antibodies

Product name

DEFA1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Positive Samples: Mouse spleen Immunogen sequence: MRTLAILAAI LLVALQAQAE PLQARADEVA AAPEQIAADI PEVVVSLAWD ESLAPKHPGS RKNMACYCRI PACIAGERRY GTCIYQGRLW AFCC

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.03 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DEFA1 in extracts of various cell lines. Samples were incubated with DEFA1 Polyclonal antibody (Product # PA5-117076) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of DEFA1 in HeLa cells using DEFA1 Polyclonal Antibody (Product # PA5-117076) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of DEFA1 in Jurkat cells. Samples were incubated with DEFA1 Polyclonal antibody (Product # PA5-117076) using a dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.