PA1-043

antibody from Invitrogen Antibodies

Targeting: DLG2 chapsyn-110, PPP1R58, PSD-93, PSD93

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-043

Provider

Invitrogen Antibodies

Product name

PSD93 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1-043 detects Post Synaptic Density 93 (PSD-93) from rat and human tissues. This antibody does not detect other synapse-associated protein family members. PA1-043 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects an ~110 kDa protein representing PSD-93 in rat brain extract. Immunohistochemical staining of PSD-93 in rat cerebellum with PA1-043 yields a pattern consistent with post-synaptic localization. The PA1-043 immunogen is a synthetic peptide corresponding to residues N(352) K L C D K P A S P R H Y S P(366) of human PSD-93. This sequence is completely conserved in human PSD-93. PA1-043 immunizing peptide (Cat. # PEP-021) is available for use in neutralization and control experiments.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Deletion of densin-180 results in abnormal behaviors associated with mental illness and reduces mGluR5 and DISC1 in the postsynaptic density fraction.

Carlisle HJ, Luong TN, Medina-Marino A, Schenker L, Khorosheva E, Indersmitten T, Gunapala KM, Steele AD, O'Dell TJ, Patterson PH, Kennedy MB
The Journal of neuroscience : the official journal of the Society for Neuroscience 2011 Nov 9;31(45):16194-207

---

Subunit- and pathway-specific localization of NMDA receptors and scaffolding proteins at ganglion cell synapses in rat retina.

Zhang J, Diamond JS
The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 Apr 1;29(13):4274-86

---

Guanylyl cyclase/PSD-95 interaction: targeting of the nitric oxide-sensitive alpha2beta1 guanylyl cyclase to synaptic membranes.

Russwurm M, Wittau N, Koesling D
The Journal of biological chemistry 2001 Nov 30;276(48):44647-52

---

Heteromultimerization and NMDA receptor-clustering activity of Chapsyn-110, a member of the PSD-95 family of proteins.

Kim E, Cho KO, Rothschild A, Sheng M
Neuron 1996 Jul;17(1):103-13

---

Cloning and characterization of postsynaptic density 93, a nitric oxide synthase interacting protein.

Brenman JE, Christopherson KS, Craven SE, McGee AW, Bredt DS
The Journal of neuroscience : the official journal of the Society for Neuroscience 1996 Dec 1;16(23):7407-15

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on membrane-enriched extracts (30 µg lysate) of SHY-SY5Y (Lane 1), and Neuro-2a (Lane 2). The blots were probed with Anti-PSD93 Rabbit Polyclonal Antibody (Product # PA1-043, 3 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 115 kDa band corresponding to PSD93 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunolocalization of PSD-93 in rat cerebellum using Product # PA1-043.