701999

antibody from Invitrogen Antibodies

Targeting: DLG2 chapsyn-110, PPP1R58, PSD-93, PSD93

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

701999

Provider

Invitrogen Antibodies

Product name

PSD93 Recombinant Rabbit Monoclonal Antibody (7H7L3)

Antibody type

Monoclonal

Antigen

Other

Description

This antibody is predicted to react with Rat, Mouse, Monkey and Chicken.

Antibody clone number

7H7L3

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on extracts (30 µg of lysate) of Rat Brain (Lane1), Mouse Brain (Lane 2), SH-SY5Y (Lane 3) Mouse Cerebellum (Lane 4), Neuro-2a (Lane 5), IMR32 (Lane 6), Caco-2 (Lane 7). The blots were probed with Chapsyn-110 Recombinant Rabbit Monoclonal Antibody (Product # 701999, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 65 kDa band corresponding to Chapsyn-110 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis was performed on fixed and permeabilized SH-SY5Y cells for detection of endogenous Chapsyn-110 using Anti-Chapsyn-110 Recombinant Rabbit Monoclonal Antibody (Product # 701999, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of Chapsyn-110 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of Chapsyn-110. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.