Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ACC-018-25UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRPC4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µL
- Concentration
- 0.4 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysisof rat brain membranes: - 1. Anti-TRPC4 Antibody (#ACC-018), (1:200). 2. Anti-TRPC4 Antibody , preincubated with TRPC4 Blocking Peptide (#BLP-CC018).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human PC3 Caucasian prostate adenocarcinoma (lanes 1 and 3) andhuman LNCaP prostate carcinoma (lanes 2 and 4) cell lysates: - 1,3. Anti-TRPC4 Antibody (#ACC-018), (1:200).2,4. Anti-TRPC4 Antibody , preincubated with TRPC4 Blocking Peptide (#BLP-CC018).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of TRPC4 in mouse cerebellum - Immunohistochemical staining ofmouse cerebellum frozen sections with Anti-TRPC4 Antibody (#ACC-018). A. TRPC4 (red) appears in Purkinje cells (arrows) and in the molecular (Mol) layer. B. Staining with mouse Anti-parvalbumin (PV)in the same brain section. C. Confocal merge of TRPC4 and PV demonstrates partial co-localization in the Purkinje and the molecular layers.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of rat brain lysate: - 1. Rat brain lysate.2.Lysate immunoprecipitated with Anti-TRPC4 Antibody (#ACC-018), (6 µg).3. Lysate immunoprecipitated with pre-immune rabbit serum. The upper arrow indicates the TRPC4 channel while the lower arrow indicates the IgG heavy chain. Western blot analysiswas performed with Anti-TRPC4 Antibody .
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of rat brain lysate: - 1. Rat brain lysate.2.Lysate immunoprecipitated with Anti-TRPC4 Antibody (#ACC-018), (6 µg).3. Lysate immunoprecipitated with pre-immune rabbit serum. The upper arrow indicates the TRPC4 channel while the lower arrow indicates the IgG heavy chain. Western blot analysiswas performed with Anti-TRPC4 Antibody .
