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antibody from Invitrogen Antibodies
Targeting: TMEM231
ALYE870, FLJ22167, JBTS20, MKS11, PRO1886
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-42686 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TMEM231 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: CHEAPRARSA RAGLPNRLPT ALFNSGFWLK RSSYEEQPTV RFQHQVLLVA Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 93%; Human: 100%; Mouse: 93%; Rabbit: 100%; Rat: 100%; Zebrafish: 79%
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references TMEM216 Deletion Causes Mislocalization of Cone Opsin and Rhodopsin and Photoreceptor Degeneration in Zebrafish.
Liu Y, Cao S, Yu M, Hu H
Investigative ophthalmology & visual science 2020 Jul 1;61(8):24
Investigative ophthalmology & visual science 2020 Jul 1;61(8):24
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human HepG2 cell lysate using an anti-FLJ22167 polyclonal antibody (Product # PA5-42686).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of human kidney tissue using an anti-FLJ22167 polyclonal antibody (Product # PA5-42686).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 8. Length of photoreceptor axonemes was reduced in knockout zebrafish. Cryosections were immunostained with axoneme marker, acetylated alpha-tubulin ( red ), and connecting ciliary region markers CC2D2A, TMEM231, and EYS ( green ). Sections were then counterstained with DAPI to visualize nuclei. ( A, B ) Acetylated alpha-tubulin ( red, arrow ) and CC2D2A ( green, arrowhead ) double immuno-labeling of 7dpf wildtype and tmem216 snyDelta175 homozygous retina. ( C, D ) Acetylated alpha-tubulin ( red, arrow ) and TMEM231 ( green, arrowhead ) double immuno-labeling of 7dpf wildtype and tmem216 snyDelta175 homozygous retina. ( E, F ) Acetylated alpha-tubulin ( red, arrow ) and EYS ( green, arrowhead ) double immuno-labeling of 7dpf wildtype and tmem216 snyDelta175 homozygous retina. Note the reduction in axoneme length in the knockout zebrafish ( F, inset ). ( J, K ) Acetylated alpha-tubulin and EYS double immuno-labeling of 14-dpf wildtype and tmem216 snyDelta175 homozygous retina. ( G-I ) Quantification of 7-dpf axoneme number and length. Note the reduction in average axoneme length ( n = 3, Student's t -test) and the shift of axoneme lengths toward lower length bins in the knockout. ( L-N ) Quantification of 14-dpf axoneme number and length ( n = 3, Student's t -test). Scale bar in K : 6 um for A-F , J, and K ; 1.5 um for insets.
