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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
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- Product number
- R31681 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- MSK1 Antibody / RPS6KA5
- Antibody type
- Polyclonal
- Description
- This highly specific MSK1 antibody is suitable for use in Western blot/Immunohistochemistry/Immunocytochemistry/Immunofluorescence applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the MSK1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of MSK1 antibody and Lane 1: human placenta; 2: rat brain; 3: (h) Jurkat; 4: (h) HeLa; 5: (h) PANC; 6: (h) A549; 7: (h) HEPG2. Expected size ~90KD
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of MSK1 antibody and recombinant human protein (0.5ng)
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HEL cell lysate, 2) mouse brain tissue lysate and 3) rat brain tissue lysate with MSK1 antibody. Expected molecular weight: ~90 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human T-47D cells with MSK1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: MSK1 antibody testing of human placenta tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P testing of mouse heart tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P testing of rat brain tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human placental tissue with MSK1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE mouse heart tissue with MSK1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat brain tissue with MSK1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
