PA5-92855

antibody from Invitrogen Antibodies

Targeting: CHAF1B CAF-1, CAF1, CAF1A, CAF1P60, MPHOSPH7, MPP7

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-92855

Provider

Invitrogen Antibodies

Product name

CAF1 p60 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: SRKNLKRPIA HLPCPGKATL AVRCCPVYFE LRPVVETGVE LMSLPYRLVF AVASEDSVLL YDTQQSFPFG YVSNIHYHTL SDISWSSDGA FLAISSTDGY CSFVTFEKDE LGIPLKEKPV LNMRTPDTAK KTKSQTHRGS SPGPRPVEGT PASRTQDPSS PGTTPPQARQ APAPTVIRDP PSITPAVKSP LPGPSEEKTL QPSSQNTKAH PSRRVTLNTL QAWSKTTPRR INLTPLKTDT PPSSVPTSVI STPSTEEIQS ETPGDAQGSP PELKRPRLDE NKGGTESLDP; Positive Samples: HeLa, 293T, MCF7, U-251MG; Cellular Location: Cytoplasm, Nucleus

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.64 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using CHAF1B Polyclonal antibody (Product # PA5-92855) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 30s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of CAF1 p60 in extracts of various cell lines using CAF1 p60 Polyclonal Antibody (Product # PA5-92855) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of CAF1 p60 in extracts of various cell lines using CAF1 p60 Polyclonal Antibody (Product # PA5-92855) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of CAF1 p60 was performed in MCF-7 cells using CAF1 p60 Polyclonal Antibody (Product # PA5-92855).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CAF1 p60 in paraffin-embedded rat fallopian tube using CAF1 p60 Polyclonal Antibody (Product # PA5-92855) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CAF1 p60 in paraffin-embedded human tonsil using CAF1 p60 Polyclonal Antibody (Product # PA5-92855) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CAF1 p60 in paraffin-embedded mouse spleen using CAF1 p60 Polyclonal Antibody (Product # PA5-92855) at a dilution of 1:200.