- 71-1200 - Invitrogen Antibodies CTNNA2 antibody

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Co-immunoprecipitation of E-cadherin, b-catenin, and a-catenin from A431 cell lysates using: IP: Rb anti-a-Catenin (lanes 1-4, Product # 71-1200) Western: Lane 1=control, Lane 2= Ms anti-E-cadherin, Lane 3= Ms anti-b-catenin, Lane 4= Ms anti-a-catenin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Flow cytometry analysis of alpha Catenin was done on SH-SY5Y cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with alpha Catenin Rabbit Polyclonal Antibody (711200, red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Co-immunoprecipitation of E-cadherin, b-catenin, and a-catenin from A431 cell lysates using: IP: Rb anti-a-Catenin (lanes 1-4, Product # 71-1200) Western: Lane 1=control, Lane 2= Ms anti-E-cadherin, Lane 3= Ms anti-b-catenin, Lane 4= Ms anti-a-catenin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 6 alpha3beta4-myc nAChRs localize to the apical cell surface and colocalize with F-actin and Arp3. Monolayers of Caco-2 cells expressing alpha3beta4-myc nAChRs were fixed, surface labeled with anti-myc antibodies, permeabilized, and colabeled with anti-alpha-catenin (A), Alexa-488 phalloidin (B), and anti-Arp3 (C) antibodies. A) Deconvoluted confocal images of the basal and apical regions of polarized Caco-2 cells show localization of alpha-catenin on the basolateral cell membrane while alpha3beta4-myc is exclusively localized to the apical cell surface. B) Deconvoluted confocal images of the basal and apical regions of polarized Caco-2 cells colabeled with Alexa-488 phalloidin show localization of F-actin in both the basolateral and apical cell surface. Higher magnification images of the apical cell surface show colocalization of alpha3beta4-myc nAChRs with F-actin (arrowheads). C) Deconvoluted confocal images of the apical region of Caco-2 cells show colocalization of alpha3beta4-myc nAChRs with Arp3 (arrowheads). Scale bar in A and B, 5 um; scale bar in B higher magnification and in C, 1 um.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Fig. 4 Plexin-B1/Plexin-B2 mediate mechanosensation through stabilization of adhesive cell-cell junctions. a Primary mouse keratinocytes were cultured with 70 uM Ca 2+ . 1.8 mM Ca 2+ was added (""high calcium""). Shown are confocal images of immunostainings for Plexin-B1 (green) and Plexin-B2 (red). Scale bar, 25 um. b , f Specific adhesion of primary mouse keratinocytes to the recombinant extracellular portions of b Plexin-B1 or Plexin-B2, and f E-cadherin (mean +- s.d. ; n = 4 mice per genotype; p = 0.0124 for Plexin-B1, p = 0.0332 for Plexin-B2; p = 0.0006 for E-cadherin; two-sided unpaired t -test). c Live cell imaging of primary mouse keratinocytes expressing E-cadherin-mRuby. Shown are representative epifluorescence still images of cell divisions. Arrows: dividing cells; arrowheads: cell-cell contacts. Time: hours:minutes format. Scale bar, 10 um. d , h , j Confocal images of the epidermal basal layer at E15.5 immunostained for d E-cadherin (red), h alpha-catenin (red), and j a-18 (=alpha-catenin tension-sensitive epitope antibody; green). Cell divisions are contoured by dashed lines. Scale bar, 10 um. e , i , k Quantification of e E-cadherin, i alpha-catenin, and k a-18 immunofluorescence intensities at cell-cell contacts between a dividing cell and its immediate neighbors (box plot with minimum, first quartile, median, third quartile and maximum). e control: n = 207 cell-cell contacts of 34 dividing cells from three mice, PlexDKO: n = 209 cell-cell contacts of 34 divi

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 3--figure supplement 1. A basal endfoot mediates planar telophase correction. ( A ) On the x-axis, divisions are grouped by orientation at anaphase onset (phi, planar = 0-30deg; oblique = 30-60deg; perpendicular = 60-90deg) and Gpsm2 1617 status. The y-axis depicts the proportion of divisions that lack (white), maintain (black/red), or lose (gray/pink) basal contact in the following hour. ~95% of planar divisions initiate and maintain basal contact; oblique divisions make and initiate contact less frequently (~75% of the time); while perpendicular divisions almost never make contact. LGN knockdown (red bars) does not alter this behavior. ( B-C ) Radial anaphase correction (theta-phi) plotted versus initial anaphase orientation. Cells where apical daughter basal contacts were detected are shown as black/red circles, while those lacking basal contacts are shown in gray/pink. In wild-type controls (left) basal contact correlates with planar reorientation, while lack of contact results in perpendicular reorientation. ( B ) Telophase correction (theta-phi) in Scramble RFP+ cells (middle panel), grouped by basal contact status. Like wild-type cells, Scramble RFP+ cells undergo telophase reorientation in a basal contact-dependent manner. ( C ) Both Gpsm2 1617 RFP+ (right panel; red) and wild-type RFP- cells (black) with basal contacts correct to planar, demonstrating that LGN is not required for this behavior. LGN knockdown cells only rarely (n = 3) lack basement membrane con

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 3. Maintenance of basal contact correlates with planar-directed telophase correction. ( A ) (top) z-projection stills from a movie of a mitotic cell as it enters anaphase (t = 0) through 60 min post-anaphase onset, depicting planar telophase correction. Epidermal-dermal boundary shown by red line. Dividing daughter pairs are outlined with yellow dashed lines. Division orientation angles are shown below (phi, telophase onset; theta, +1h). (bottom), xz en face views at same timepoints. Yellow and white arrowheads indicate plane of optical section for apical and basal daughters, respectively. In most cases, planar correction is preceded by maintenance of basement membrane contact (open arrowheads), which are most apparent in the en face basal focal plane, where they appear as small membrane circles. ( B ) Data from Figure 1G,H sorted based on presence or absence of basal contact. Connecting lines demonstrate that oblique-dividing daughters retaining basal contact correct towards a planar orientation, while those losing contact correct towards perpendicular. ( C ) Data from ( B ) demonstrating that the degree of correction correlates with initial anaphase orientation. ( D ) Whole mount imaging of wild-type E16.5 epidermis stained with phalloidin and phosphorylated myosin-light chain 2 (pMLC2). Orthogonal views (top) of DAPI highlight oblique division orientation. The basal endfoot observed in live imaging of telophase correction (see panel A) can be observed in the basal e

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 4--figure supplement 1. The alpha-E-catenin/vinculin/afadin complex demonstrates reciprocal regulation to form mature adherens junctions in vitro. ( A ) Quantification of alpha18 (left) and alpha-E-catenin (right) fluorescence intensity in 8h Ca 2+ -shift assays in Scramble and Vcl shRNA expressing primary keratinocytes. ( B ) Time-course of junction formation in wild-type primary keratinocytes 30 min (left) and 8h (right) after the addition of 1.5 mM Ca 2+ , stained for E-cadherin (green), afadin (red), and phalloidin (gray). Between early and late timepoints, junctions transform from punctate to linear and actin becomes tightly associated with the junction. ( C ) Quantification of E-cad continuity along junction length in wild-type primary keratinocytes at 30 min and 8h timepoints. ( D ) Primary mouse keratinocytes after 8h Ca 2+ shift--labeled with afadin (red), E-cadherin (green) and actin (phalloidin, gray)--which accumulate in linear bands at cell-cell junctions in Scramble control cells. Yellow boxed region shown at high magnification to the right; n indicates junctions evaluated. Vcl and Ctnna1 knockdown cells show defects in linear actin accumulation as well as afadin recruitment to AJs, quantified in ( E ). ( F ) Scramble, Afdn 2711 , Afdn fl/fl , and Afdn cKO keratinocytes after 8h Ca 2+ shift and stained for alpha18 (red), alpha-E-catenin (green), and vinculin (gray). ( G-H ) fluorescence intensity quantification of alpha18 ( G ) and alpha-E-catenin ( H ) i

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 4. Vinculin and afadin regulate alpha-E-catenin conformation and AJ linkage to the actin cytoskeleton. ( A ) Stable primary murine keratinocytes cell lines grown in the presence of high (1.5 mM) Ca 2+ for 8h form nascent cell-cell adhesions, stained for total alpha-E-catenin (green); open, ""tensile"" alpha-E-catenin (alpha18, red); and vinculin (gray). Single junction magnifications (yellow dashed region) shown below, demonstrate that Vcl knockdown results in a reduced alpha18: alpha-E-catenin ratio, quantified in ( B,C ). ( B ) Fluorescence intensity quantification of junctional vinculin in Scramble and Ctnna1 knockdown keratinocytes. Loss of Ctnna1 reduces vinculin accumulation in nascent AJs. ( C ) Quantification of alpha18:alpha-E-catenin fluorescence intensity ratio in Scramble and two independent Vcl shRNA cell lines. Vcl knockdown reduces the proportion of alpha-E-catenin in the open conformation. ( D ) Primary mouse keratinocytes after 8h Ca 2+ shift--labeled with phalloidin (red) and E-cad (green)--which accumulate in linear bands at cell-cell junctions in Scramble control cells. Yellow boxed region shown at high magnification below; n indicates junctions evaluated. Vcl and Ctnna1 knockdown cells show defects in linear actin accumulation and immature ""zipper"" junctional morphology. ( E ) Junction continuity quantification based on % of junction length above threshold for E-cad (see Materials and methods). Loss of Vcl or Ctnna1 reduces junction continuity. (

71-1200

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