MA1-2000

antibody from Invitrogen Antibodies

Targeting: CTNNA2 CAP-R, CT114

Western blot

Immunoprecipitation

Antibody data

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Validation data

Product number: MA1-2000 - Provider product page
Provider: Invitrogen Antibodies
Product name: alpha Catenin Monoclonal Antibody (1G5)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA1-2000 detects alpha catenin from human samples. MA1-2000 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~102 kDa protein representing alpha catenin from scc-1 cell extract. In Western blot, this antibody did not cross-react with mouse samples. MA1-2000 can also be used in immunoprecipitation and immunofluorescence procedures. The MA1-2000 immunogen is a 90 kDa MBP/alpha catenin fusion protein purified via an amylose column.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: 1G5
Vial size: 100 μg
Concentration: 1 mg/mL
Storage: -20°C, Avoid Freeze/Thaw Cycles

CTNNA2 protein structure - MA1-2000 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of alpha Catenin using Catenin alpha Monoclonal Antibody (1G5) (Product # MA1-2000) shows staining in A2058 Cells. Catenin alpha (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Catenin alpha (Product # MA1-2000) at a dilution of 1:20 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552 for GAR, Product # 35503 for GAM). Images were taken at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of alpha Catenin using Catenin alpha Monoclonal Antibody (1G5) (Product # MA1-2000) shows staining in Hela Cells. Catenin alpha (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Catenin alpha (Product # MA1-2000) at a dilution of 1:20 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552 for GAR, Product # 35503 for GAM). Images were taken at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of alpha Catenin using Catenin alpha Monoclonal Antibody (1G5) (Product # MA1-2000) shows staining in A2058 Cells. Catenin alpha (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Catenin alpha (Product # MA1-2000) at a dilution of 1:20 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552 for GAR, Product # 35503 for GAM). Images were taken at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of alpha Catenin using Catenin alpha Monoclonal Antibody (1G5) (Product # MA1-2000) shows staining in Hela Cells. Catenin alpha (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Catenin alpha (Product # MA1-2000) at a dilution of 1:20 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552 for GAR, Product # 35503 for GAM). Images were taken at 60X magnification.