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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- MA5-24344 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-EMP Monoclonal Antibody (730340)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 730340
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, C2C12 mouse myoblast cell line, NR8383 rat alveolar macrophage cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 0.5 µg/mL of mouse Anti-human/mouse/Rat EMP/MAEA Monoclonal Antibody (Product # MA5-24344) followed by HRP-conjugated Anti-mouse IgG Secondary Antibody. Specific bands were detected for EMP/MAEA at approximately 36-45 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of EMP/MAEA was detected in immersion fixed Jurkat human acute T cell leukemia cell line using mouse Anti-human/mouse/Rat EMP/MAEA Monoclonal Antibody (Product # MA5-24344) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the 557-conjugated Anti-mouse IgG Secondary Antibody (red, upper pane and counterstained with DAPI (blue, lower panel). Specific staining was localized to cell surfaces and cytoplasm.
