PA5-47914

antibody from Invitrogen Antibodies

Targeting: MAEA EMP, GID9, HLC-10, p44EMLP

Antibody data

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Validation data

Product number: PA5-47914 - Provider product page
Provider: Invitrogen Antibodies
Product name: EMP Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Reconstitute in sterile PBS to a final concentration of 0.2 mg/mL.
Reactivity: Human, Mouse, Rat
Host: Sheep
Isotype: IgG
Vial size: 100 µg
Concentration: 0.2 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

MAEA protein structure - PA5-47914 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis from lysates of C2C12 mouse myoblast cell line, Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-human EMP/MAEA Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47914) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for EMP/MAEA at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of EMP in C2C12 mouse myoblast cell line, Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, and NR8383 rat alveolar macrophage cell line. Samples were incubated in EMP polyclonal antibody (Product # PA5-47914) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. A specific band was detected for EMP/MAEA at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of EMP/MAEA was performed by loading whole cell lysate in 1X LDS sample buffer with 2-ME from 5 x 105 Cas9-expressing mouse transformed pre-B cells. Samples were loaded onto a 4-12 % Bis-Tris polyacrylamide gel (Product # NP0336BOX). Proteins were transferred to nitrocellulose membrane by wet/tank transfer. Membrane was blocked in 5% milk/TBST. Target was detected at approximately 40 kDa using a polyclonal anti-EMP/Maea antibody (Product # PA5-47914) at a dilution of 0.5mg/ml in 5% milk/TBST at 4C overnight, followed by a secondary antibody HRP-anti-rabbit at a dilution of 1:5000 at room temperature for 1 hour. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # PI-32209). Data courtesy of Thermo Scientific KOL Program.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of EMP in C2C12 mouse myoblast cell line, Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, and NR8383 rat alveolar macrophage cell line. Samples were incubated in EMP polyclonal antibody (Product # PA5-47914) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. A specific band was detected for EMP/MAEA at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions.