orb150337

antibody from Biorbyt

Targeting: NOTCH1 TAN1

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

orb150337

Provider

Biorbyt

Product name

Notch1 Antibody: Biotin

Antibody type

Monoclonal

Description

Mouse monoclonal to Notch1 (Biotin). Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane..

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Biotin

Isotype

IgG

Antibody clone number

N253/32 (Formerly sold as S253-32)

Vial size

100 ug

Concentration

1 mg/ml

Storage

Conjugated antibodies should be stored according to the product label