10062-2-AP

antibody from Proteintech Group

Targeting: NOTCH1 TAN1

Provider product page for 10062-2-AP

Western blot

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Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [77]
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Product number: 10062-2-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#10062-2-AP, RRID:AB_2153338
Product name: Notch1 antibody
Antibody type: Polyclonal
Description: KD/KO validated Notch1 antibody (Cat. #10062-2-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, WB,ELISA.
Reactivity: Human, Mouse
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

NOTCH1 protein structure - 10062-2-AP shown in red.

Submitted references Salidroside targets the Notch1/Hes5 axis to reconstruct the molecular innate immune-vascular network and correlates with repair after ischemic stroke.

Zeng J, Huang X, Zeng J, Huang Q, Song W, Xie Y, Liang G, Wei Q, Lan Y, Zou D, Li R, Gu L
Frontiers in immunology 2026;17:1756559

The Protective Effects of Dendrobine on LPS-Induced Neuroinflammation and Related Mechanisms Based on Microglial M1/M2 Polarization.

Cui J, Zhang X, Sun J, Liu J, Fan B, Wang F, Lu C
Nutrients 2026 Mar 5;18(5)

LIFUS-driven engineered bacteria reprogram immunosuppressive niches via mechano-NOTCH signaling.

Lin L, Li X, Guo W, Xie J, Li X, Guan X, Zhao C, Yin H, Xu H
Cell reports. Medicine 2026 Mar 17;7(3):102658

The deubiquitinase USP28 promotes esophageal squamous cell carcinoma proliferation by stabilizing ΔNp63 protein.

Cai C, Cheng N, Luo H, Guan H, Peng J, Zhang P, Chen X, Fang Y
Cellular signalling 2026 Jul;143:112489

Therapeutic efficacy and concomitant toxicity of Hongsheng Dan in enhancing healing of chronic refractory wounds in rats.

Yang J, Gan Q, Yang Y, Liu C, Zhang T, Xu J, Wang J, Huang Q
Journal of ethnopharmacology 2026 Jan 30;355(Pt A):120671

Network pharmacology and molecular docking reveal the multi-target mechanisms of Paeoniae Radix Rubra in functional angiogenesis: Synergistic regulation of ADAM10-NOTCH and VEGFR2-PI3K/AKT signaling.

Zhang R, Hu R, Wang Y, Jiang H, Aheniyazi A, Tao J, Li J, Yang Y
Journal of ethnopharmacology 2026 Jan 30;355(Pt A):120532

GATA6 inhibits the periodontitis progression through regulating the Notch signaling pathway.

Li WW, Li QW, Yu J, Sui XN, Wang MM, Yin NN, Liu X
Odontology 2026 Apr;114(2):893-902

CRTC1 enhances PD-L1-mediated tumor immunosuppression in non-small cell lung cancer via the Notch1/Akt signaling pathway.

Feng X, Shi Y, Yuan F, Hu Y, Tang X, Zhang W, Gan J, Sun L, Cao L
Frontiers in immunology 2025;16:1658679

Androgen receptor inhibitor ameliorates pulmonary arterial hypertension by enhancing the apoptosis level through suppressing the Notch3/Hes5 pathway.

Sun J, Lin J, Yin D, Pan Z, Ye Y, Wang Y, Wang X, Guo Q
Frontiers in pharmacology 2025;16:1572489

Regulating osteogenic differentiation of bone marrow mesenchymal stem cells by mTORC1 signaling pathway inhibits bone defect repair in mice.

Huang Z, Zhang Z, Yang C, Mai Q, Li R, Li S, Fan S, Yang J
Journal of orthopaedic surgery and research 2025 Sep 29;20(1):860

Experimental validation, molecular docking, proteomic analysis and network pharmacology reveal Morroniside promoting neurogenesis and improving cognitive impairment via Notch1/Hes1 pathway in intracerebroventricular-streptozotocin mice.

Tan Q, Liu Z, Zheng J, Wang Y, Wu H, Wang Q, Liang Y
Journal of ethnopharmacology 2025 Sep 25;353(Pt A):120322

Bruceine D inhibits CAF-promoted angiogenesis of breast cancer via suppressing IL-6-mediated activation of the STAT3/Notch1/VEGFR2 axis.

Ma J, Cao D, Zhang Y, Sun Y, Wu Y, Wang J
European journal of pharmacology 2025 Sep 15;1003:177994

Effect of monochromatic light on self-renewal and differentiation of intestinal stem cells in broilers.

Tao X, Hu G, Wang Z, Chen Y, Cao J
Poultry science 2025 Oct;104(10):105499

Lactoferrin and Osteopontin Cooperatively Promote Intestinal Epithelial Maturation in Neonatal Mice by Activating the Brg1/Notch1/Hes1 Pathway.

Zhang W, Li C, Bi R, Lu Y, Zhang Y, Shi C, Qiao Z, Sun Y, Chen J, Wang P, Wang R, Ren F, Li Y
Nutrients 2025 Oct 8;17(19)

MYT1L promotes autophagy to ameliorate amyloid-β pathology and cognitive deficits in Alzheimer's disease via Notch signaling pathway.

Zhang M, Hai S, Gao X, Cui Z, Yang T, Wang A, Lin J, Sun X
Experimental neurology 2025 Oct 6;395:115492

Ershen Dan attenuates atherosclerosis by modulating the NOTCH1/NF-κB/NLRP3 signaling pathway to suppress M1 macrophage polarization.

Zhao W, Wang H, Peng S, Wei Q, Zhang L, Li Y, Yang W
Chinese medicine 2025 Oct 23;20(1):178

Macrophage Notch1 drives septic cardiac dysfunction by impairing mitophagy and promoting NLRP3 activation.

Zheng Y, Lin J, Wan G, Gu X, Ma J
Biology direct 2025 May 26;20(1):65

ALKBH5 promotes cardiac fibroblasts pyroptosis after myocardial infarction through Notch1/NLRP3 pathway.

Cui LG, Wang SH, Komal S, Yin JJ, Zhai MM, Zhou YJ, Yu QW, Wang C, Wang P, Wang ZM, Zafar AM, Shakeel M, Zhang LR, Han SN
Cellular signalling 2025 Mar;127:111574

α-Lipoic Acid Ameliorates Arsenic-Induced Lipid Disorders by Promoting Peroxisomal β-Oxidation and Reducing Lipophagy in Chicken Hepatocyte.

Zhao Y, Guo M, Pei T, Shang C, Chen Y, Zhao L, Lu Y, Liang C, Wang J, Zhang J
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Mar;12(11):e2413255

Targeting DUSP3 promotes cell senescence by activating the notch1 pathway to treat hepatocellular carcinoma.

Zhang WJ, Peng JL, Dai DF, Huang C, Chen XP
Tissue & cell 2025 Jun;94:102781

Advanced Strategies in Bone Tissue Engineering: "Membrane-Jelly" Hydrogel System to Improve Bone Marrow Stem Cell Osteogenic Differentiation and Bone Regeneration.

Ren Y, Xu Z, Xu Y, Xu Y, Chen Y, Chen M, Duan R, Yuan C
ACS applied materials & interfaces 2025 Jun 18;17(24):34982-34996

Combined therapy with DR5-targeting antibody-drug conjugate and CDK inhibitors as a strategy for advanced colorectal cancer.

Zhou D, Tang E, Wang W, Xiao Y, Huang J, Liu J, Zheng C, Zhang K, Hu R, Wang F, Xiong P, Chu X, Li W, Liu D, Zeng X, Zheng D, Wang L, Zheng Y, Zhang S
Cell reports. Medicine 2025 Jun 17;6(6):102158

Qishen Granule protects against myocardial ischemia by promoting angiogenesis through BMP2-Dll4-Notch1 pathway.

Hong Y, Wang H, Xie H, Zhong X, Chen X, Yu L, Zhang Y, Zhang J, Wang Q, Tang B, Lu L, Guo D
Chinese herbal medicines 2025 Jan;17(1):139-147

Correcting mitochondrial loss mitigates NOTCH1-related aortopathy in mice.

Tang Y, Zhang J, Fang Y, Zhu K, Zhu J, Huang C, Xie Z, Zhang S, Ma W, Yan G, Liu S, Liu X, Han W, Xin Y, Yang C, Abudupataer M, Zhou P, He C, Lai H, Wang C, Liu Y, Lan F, Ye D, Yu FX, Xu Y, Zhang W
Nature cardiovascular research 2025 Feb;4(2):235-247

HSF1 Increases EOGT-Mediated Glycosylation of Notch1 to Promote IL-1β-Induced Inflammatory Injury of Chondrocytes.

Huang Y, Pan W, Bao H, Sun X, Xu C, Ma J
Cartilage 2025 Dec;16(4):486-494

Butylphthalide enhances neurorestoration following ischemic stroke by restructuring microvasculature through vimentin modulation.

Xiao H, Xie H, Qin M, Zuo C, Zheng R, Zhang S, Sun S, Ye J, Wang M, Tian Y, Cao L, Sun G
Journal of advanced research 2025 Dec 7;

Local therapeutic platform prevents postsurgical GBM recurrence by diminishing GICs and reshaping immunosuppressive microenvironment.

Zhu M, Yang M, Li R, Hu X, Chen J, Xu L, Chen S, Li T, Wang J, Zheng B, He H
Nature communications 2025 Dec 18;17(1):636

CSF2 promotes chemoresistance in colorectal cancer by regulating Notch pathway.

Zhou H, Gao Z, Wu X, Wang Y, Zhang L
Discover oncology 2025 Apr 9;16(1):495

Gualou Guizhi Granule inhibits microglia-mediated neuroinflammation to protect against neuronal apoptosis in vitro and in vivo.

Li X, Huang H, Li Y, Feng Y, Wang J, Luo S, Chen Y, Zhang Y, Yan G, Nan L
Frontiers in immunology 2024;15:1527986

Expression and clinical significance of Numb and Notch-1 proteins between tissue of colon cancer and regional lymph node metastases.

Ma J, Zhen J, Yang N, Meng C, Lian Y
Frontiers in oncology 2024;14:1467517

The impact of NUMB on chicken abdominal adipogenesis: A comprehensive analysis.

Wang Z, Zhao M, Su Y, Zhao Q, Ma Z, Yue Q, Zhu Z, Zhang L, Hou Z, Li H
International journal of biological macromolecules 2024 Oct;278(Pt 3):134904

Protective Effects of Gastrodin Against Gentamicin-Induced Vestibular Damage by the Notch Signaling Pathway.

Jiang W, Li F, Xu H, Cao M, Xiao B, Gong K, Ma J, Zhang W, Tang X, Liu F, Yu S
Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology 2024 Oct 1;45(9):1059-1067

ZNF217-activated Notch signaling mediates sulforaphane-suppressed stem cell properties in colorectal cancer.

Wang M, Tang L, Chen S, Wang L, Wu J, Zhong C, Li Y, Chen Y
The Journal of nutritional biochemistry 2024 Mar;125:109551

Modulating Th1/Th2 drift in asthma-related immune inflammation by enhancing bone mesenchymal stem cell homing through targeted inhibition of the Notch1/Jagged1 signaling pathway.

Kun W, Xiaomei C, Lei Y, Huizhi Z
International immunopharmacology 2024 Mar 30;130:111713

Distinct cellular microenvironment with cytotypic effects regulates orderly regeneration of vascular tissues.

Wang J, Yang X, Xu M, Liu H, Liu L, Tan Z
Materials today. Bio 2024 Jun;26:101033

Linarin Ameliorates Restenosis After Vascular Injury in Type 2 Diabetes Mellitus via Regulating ADAM10-Mediated Notch Signaling Pathway.

Jiang A, Liu L, Wang J, Liu Y, Deng S, Jiang T
Cardiovascular toxicology 2024 Jun;24(6):587-597

ZEB2 alleviates Hirschsprung's-associated enterocolitis by promoting the proliferation and differentiation of enteric neural precursor cells via the Notch-1/Jagged-2 pathway.

Feng Y, Zhou C, Zhao F, Ma T, Xiao Y, Peng K, Xia R
Gene 2024 Jun 20;912:148365

Ginsenoside Rg5 inhibits lipid accumulation and hepatocyte apoptosis via the Notch1 signaling pathway in NASH mice.

Li N, Zhu C, Fu R, Ma X, Duan Z, Fan D
Phytomedicine : international journal of phytotherapy and phytopharmacology 2024 Feb;124:155287

NOTCH1 as a Negative Regulator of Avian Adipocyte Differentiation: Implications for Fat Deposition.

Wang Z, Su Y, Zhao M, Ma Z, Li J, Hou Z, Li H
Animals : an open access journal from MDPI 2024 Feb 9;14(4)

Astragalus Polysaccharide Promotes Neuronal Injury Repair via the Notch1/NFκB Signaling Axis in the Ventroposterior Thalamic Nucleus in Rats with Focal Cerebral Ischemia.

Li W, Miao H, Nie Z, Wu F, Li H
Journal of integrative neuroscience 2024 Feb 18;23(2):34

S-Nitrosylation of NOTCH1 Regulates Mesenchymal Stem Cells Differentiation Into Hepatocyte-Like Cells by Inhibiting Notch Signalling Pathway.

Wang X, Xu Y, Wang Y, Tang X, Zhou X, Lu W, Chen W, Li L, Zhou L, Ye J
Journal of cellular and molecular medicine 2024 Dec;28(23):e70274

The bone-protective benefits of kaempferol combined with metformin by regulation of osteogenesis-angiogenesis coupling in OVX rats.

Zhang Z, Xu W, Zhang Z, Chen X, Jin H, Jiang N, Xu H
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2024 Apr;173:116364

B Lymphocytes Balance Th1/Th2 to Participate in Fibrotic Hypersensitivity Pneumonia Induced by Pigeon Shedding.

Wu C, Ding W, Li Y, Wang W, Lian Z, Muhataer X, Zhou Y, Li Q, Yang X
International archives of allergy and immunology 2023;184(2):122-131

Adiponectin inhibits TGF-β1-induced skin fibroblast proliferation and phenotype transformation via the p38 MAPK signaling pathway.

Wang X, Yan X, Huang F, Wu L
Open life sciences 2023;18(1):20220679

Jiawei Danxuan Koukang Alleviates Arecoline Induced Oral Mucosal Lesions: Network Pharmacology and the Combined Ultra-High Performance Liquid Chromatography (UPLC) and Mass Spectrometry (MS).

Zhou L, Tan J, Dai Y, Zhu K, Xiao Y, Wu D, Wang Z, Tan Y, Qin Y
Drug design, development and therapy 2023;17:3085-3101

MOGS promotes stemness acquisition and invasion via enhancing NOTCH1-glycosylation dependent NOTCH pathway in colorectal cancer.

Zhou S, Huang H, Zheng Z, Zheng K, Xie L
American journal of cancer research 2023;13(12):5996-6010

PRDM16 from hepatic stellate cells-derived extracellular vesicles promotes hepatocellular carcinoma progression.

Sun C, Xu W, Xia Y, Wang S
American journal of cancer research 2023;13(11):5254-5270

The Circadian System Is Essential for the Crosstalk of VEGF-Notch-mediated Endothelial Angiogenesis in Ischemic Stroke.

Zhang Y, Zhao X, Guo C, Zhang Y, Zeng F, Yin Q, Li Z, Shao L, Zhou D, Liu L
Neuroscience bulletin 2023 Sep;39(9):1375-1395

PCSK9 Inhibition Regulates Infarction-Induced Cardiac Myofibroblast Transdifferentiation via Notch1 Signaling.

Wu C, Lin D, Ji J, Jiang Y, Jiang F, Wang Y
Cell biochemistry and biophysics 2023 Jun;81(2):359-369

Expression of type VI collagen α3 chain in canine mammary carcinomas.

Araki M, Noguchi S, Kubo Y, Yasuda A, Koh M, Otsuka H, Yokosuka M, Soeta S
Research in veterinary science 2023 Jun;159:171-182

Thallium exposure induces changes in B and T cell generation in mice.

Li D, Li L, Yao H, Su Q, Ye J
Toxicology 2023 Jun 15;492:153532

Ginsenoside Rg1 promotes astrocyte-to-neuron transdifferentiation in rat and its possible mechanism.

Shen K, Wu D, Sun B, Zhu Y, Wang H, Zou W, Ma Y, Lu Z
CNS neuroscience & therapeutics 2023 Jan;29(1):256-269

Notch1 signalling controls the differentiation and function of myeloid-derived suppressor cells in systemic lupus erythematosus.

Li X, Fei F, Yao G, Yang X, Geng L, Wang D, Gao Y, Hou Y, Sun L
Immunology 2023 Jan;168(1):170-183

Vasorin-containing small extracellular vesicles retard intervertebral disc degeneration utilizing an injectable thermoresponsive delivery system.

Liao Z, Ke W, Liu H, Tong B, Wang K, Feng X, Hua W, Wang B, Song Y, Luo R, Liang H, Zhang W, Zhao K, Li S, Yang C
Journal of nanobiotechnology 2022 Sep 19;20(1):420

Siah1 promotes the proliferation of NSCLC cells through ubiquitinating and stabilizing Notch1.

Liu Y, Li Q, Geng L, Zhou X, Zhou X, Dong Y, Zhang Y
Experimental cell research 2022 Oct 1;419(1):113305

RHBDL2 promotes the proliferation, migration, and invasion of pancreatic cancer by stabilizing the N1ICD via the OTUD7B and activating the Notch signaling pathway.

Chen S, Cai K, Zheng D, Liu Y, Li L, He Z, Sun C, Yu C
Cell death & disease 2022 Nov 9;13(11):945

The effect of mitochondrial fusion on chondrogenic differentiation of cartilage progenitor/stem cells via Notch2 signal pathway.

Moqbel SAA, Zeng R, Ma D, Xu L, Lin C, He Y, Ma C, Xu K, Ran J, Jiang L, Wu L
Stem cell research & therapy 2022 Mar 25;13(1):127

A homogalacturonan from Lonicera japonica Thunb. disrupts angiogenesis via epidermal growth factor receptor and Delta-like 4 associated signaling.

Liao W, Hu X, Du Z, Wang P, Ding K
Glycoconjugate journal 2022 Dec;39(6):725-735

miR-141-3p regulates saturated fatty acid-induced cardiomyocyte apoptosis through Notch1/PTEN/AKT pathway via targeting PSEN1.

Xin X, Duan L, Yang H, Yu H, Bao Y, Jia D, Wu N, Qiao Y
Environmental toxicology 2022 Apr;37(4):741-753

Screening and identification of NOTCH1, CDKN2A, and NOS3 as differentially expressed autophagy-related genes in erectile dysfunction.

Luo C, Zhou X, Wang L, Zeng Q, Fan J, He S, Zhang H, Wei A
PeerJ 2021;9:e11986

Lipoxin A4 regulates microglial M1/M2 polarization after cerebral ischemia-reperfusion injury via the Notch signaling pathway.

Li QQ, Ding DH, Wang XY, Sun YY, Wu J
Experimental neurology 2021 May;339:113645

Liraglutide regulates proliferation, differentiation, and apoptosis of preosteoblasts through a signaling network of Notch/Wnt/Hedgehog signaling pathways.

Hou HW, Xue P, Wang Y, Li YK
European review for medical and pharmacological sciences 2020 Dec;24(23):12408-12422

miR-30b suppresses the progression of breast cancer through inhibition of the PI3K/Akt signaling pathway by targeting Derlin-1.

Zhou J, Xiang AZ, Guo JF, Cui HD
Translational cancer research 2019 Feb;8(1):180-190

Epithelial-mesenchymal transition induced cancer-stem-cell-like characteristics in hepatocellular carcinoma.

Jing L, Ruan Z, Sun H, Li Q, Han L, Huang L, Yu S, Wang Y, Guo H, Jiao M
Journal of cellular physiology 2019 Aug;234(10):18448-18458

Engagement of circular RNA HECW2 in the nonautophagic role of ATG5 implicated in the endothelial-mesenchymal transition.

Yang L, Han B, Zhang Y, Bai Y, Chao J, Hu G, Yao H
Autophagy 2018;14(3):404-418

Notch signaling inhibitor DAPT provides protection against acute craniocerebral injury.

Zhang HM, Liu P, Jiang C, Jin XQ, Liu RN, Li SQ, Zhao Y
PloS one 2018;13(2):e0193037

POFUT1 promotes colorectal cancer development through the activation of Notch1 signaling.

Du Y, Li D, Li N, Su C, Yang C, Lin C, Chen M, Wu R, Li X, Hu G
Cell death & disease 2018 Sep 24;9(10):995

A gene mutation in RNA-binding protein 10 is associated with lung adenocarcinoma progression and poor prognosis.

Yin LL, Wen XM, Li M, Xu YM, Zhao XF, Li J, Wang XW
Oncology letters 2018 Nov;16(5):6283-6292

Potential role of the Jagged1/Notch1 signaling pathway in the endothelial-myofibroblast transition during BLM-induced pulmonary fibrosis.

Yin Q, Wang W, Cui G, Yan L, Zhang S
Journal of cellular physiology 2018 Mar;233(3):2451-2463

The Involvement of Notch1-RBP-Jk/Msx2 Signaling Pathway in Aortic Calcification of Diabetic Nephropathy Rats.

Gong C, Li L, Qin C, Wu W, Liu Q, Li Y, Gan L, Ou S
Journal of diabetes research 2017;2017:8968523

Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling.

Liu M, Liang K, Zhen J, Zhou M, Wang X, Wang Z, Wei X, Zhang Y, Sun Y, Zhou Z, Su H, Zhang C, Li N, Gao C, Peng J, Yi F
Nature communications 2017 Sep 4;8(1):413

Fbw7 regulates apoptosis in activated B-cell like diffuse large B-cell lymphoma by targeting Stat3 for ubiquitylation and degradation.

Yao S, Xu F, Chen Y, Ge Y, Zhang F, Huang H, Li L, Lin D, Luo X, Xu J, Luo D, Zhu X, Liu Y
Journal of experimental & clinical cancer research : CR 2017 Jan 10;36(1):10

Thymosin beta 4 silencing suppresses proliferation and invasion of non-small cell lung cancer cells by repressing Notch1 activation.

Huang D, Wang S, Wang A, Chen X, Zhang H
Acta biochimica et biophysica Sinica 2016 Sep;48(9):788-94

Alterations of tumor microenvironment by carbon monoxide impedes lung cancer growth.

Nemeth Z, Csizmadia E, Vikstrom L, Li M, Bisht K, Feizi A, Otterbein S, Zuckerbraun B, Costa DB, Pandolfi PP, Fillinger J, Döme B, Otterbein LE, Wegiel B
Oncotarget 2016 Apr 26;7(17):23919-32

TRPC6 specifically interacts with APP to inhibit its cleavage by γ-secretase and reduce Aβ production.

Wang J, Lu R, Yang J, Li H, He Z, Jing N, Wang X, Wang Y
Nature communications 2015 Nov 19;6:8876

Neuropathological and biochemical assessments of an Alzheimer's disease patient treated with the γ-secretase inhibitor semagacestat.

Roher AE, Maarouf CL, Kokjohn TA, Whiteside CM, Kalback WM, Serrano G, Belden C, Liebsack C, Jacobson SA, Sabbagh MN, Beach TG
American journal of neurodegenerative disease 2014;3(3):115-33

Notch1 promotes hepatitis B virus X protein-induced hepatocarcinogenesis via Wnt/β-catenin pathway.

Sun Q, Wang R, Luo J, Wang P, Xiong S, Liu M, Cheng B
International journal of oncology 2014 Oct;45(4):1638-48

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The Notch1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of mouse Notch1. This antibody recognizes human antigen. The Notch1 antibody has been validated for the following applications: ELISA, WB analysis.
Appendix: 10062-2-AP.pdf

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