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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- PA5-116436 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- P2X6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive controls: Jurkat cell lysate, K562 cell lysate, rat skeletal muscle tissue, rat brain tissue, mouse skeletal muscle tissue, mouse heart tissue, SH-SY5Y.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Flow cytometric analysis of P2X6 in SH-SY5Y cells using a P2X6 polyclonal antibody (Product#PA5-116436). The cells were fixed, permeabilize, and stained with the primary antibody (1:50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1:1000 dilution for 30 minutes. The control was not incubated with the primary antibody (black).
