MA5-24346

antibody from Invitrogen Antibodies

Targeting: HAO1 GOX, GOX1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-24346

Provider

Invitrogen Antibodies

Product name

HAO1 Monoclonal Antibody (732410)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

732410

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis from lysates of human liver tissue, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 0.2 µg/mL of mouse Anti-human/mouse/Rat Hydroxyacid Oxidase-1/HAO-1 Monoclonal Antibody (Product # MA5-24346) followed by HRP-conjugated Anti-mouse IgG Secondary Antibody. A specific band was detected for Hydroxyacid Oxidase-1/HAO-1 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Liver (Lane 1), Rat Liver (Lane 2), Mouse Spleen (Lane 3), Mouse Brain (Lane 4) and Rat Brain (Lane 5). The blot was probed with Anti-HAO1 Monoclonal Antibody (Product # MA5-24346, 0.5µg/ml) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 40 kDa band corresponding to HAO1 was observed in Mouse, Rat Liver in comparison to other tissues which are reported to be negative for HAO1 expression.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HAO1 in human liver tissue, mouse liver tissue, and rat liver tissue. Samples were incubated in HAO1 monoclonal antibody (Product # MA5-24346) using a dilution of 0.2 µg/mL followed by a HRP-conjugated Anti-Mouse IgG secondary antibody. A specific band was detected for Hydroxyacid Oxidase‚1/HAO‚1 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions.