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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [3]
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Validation data
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- Product number
- TA501957 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501957, RRID:AB_11123964
- Product name
- HAO1 mouse monoclonal antibody, clone OTI5F4 (formerly 5F4)
- Antibody type
- Monoclonal
- Description
- HAO1 mouse monoclonal antibody, clone OTI5F4 (formerly 5F4)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- HAO1
- Isotype
- IgG
- Antibody clone number
- OTI5F4
- Vial size
- 100 µl
- Concentration
- 0.49 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HAO1 (RC216834, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HAO1.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC216834 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HAO1 antibody(TA501957), and then analyzed by flow cytometry.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-HAO1 antibody(TA501957),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-HAO1 antibody(TA501957),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
