23119-MSM1-P1

antibody from Invitrogen Antibodies

Targeting: HIC2 HRG22, KIAA1020, ZBTB30, ZNF907

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

23119-MSM1-P1

Provider

Invitrogen Antibodies

Product name

HIC2 (Hypermethylated in cancer 2) (Transcriptional Repressor) Monoclonal Antibody (PCRP-HIC2-1B1)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

PCRP-HIC2-1B1

Vial size

100 µg

Concentration

200 µg/mL

Storage

4° C, do not freeze

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of PFA-fixed HeLa cells stained using HIC2 Mouse Monoclonal Antibody (PCRP-HIC2-1B1) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of PFA-fixed HeLa cells. HIC2 Mouse Monoclonal Antibody (PCRP-HIC2-1B1) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Analysis of Protein Array containing more than 19,000 full-length human proteins using HIC2-Monospecific Mouse Monoclonal Antibody (PCRP-HIC2-1B1). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the diFerence (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SDS-PAGE analysis Purified HIC2 Mouse Monoclonal Antibody (PCRP-HIC2-1B1). Confirmation of Purity and Integrity of Antibody.